验证数据展示
经过测试的应用
| Positive WB detected in | Jurkat cells, NIH/3T3 cells |
| Positive FC (Intra) detected in | U2OS cells |
Planning an IHC experiment? We recommend our IHCeasy RAB9A Ready-To-Use IHC Kit. RAB9A primary antibody included.
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Western Blot (WB) | WB : 1:2000-1:10000 |
| Flow Cytometry (FC) (INTRA) | FC (INTRA) : 0.25 ug per 10^6 cells in a 100 µl suspension |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
84129-5-RR targets RAB9A in WB, FC (Intra), ELISA applications and shows reactivity with human samples.
| 经测试应用 | WB, FC (Intra), ELISA Application Description |
| 经测试反应性 | human |
| 免疫原 | RAB9A fusion protein Ag1969 种属同源性预测 |
| 宿主/亚型 | Rabbit / IgG |
| 抗体类别 | Recombinant |
| 产品类型 | Antibody |
| 全称 | RAB9A, member RAS oncogene family |
| 别名 | RAB9, Ras-related protein Rab-9A, Ras related protein Rab 9A, 241372F8 |
| 计算分子量 | 201 aa, 23 kDa |
| 观测分子量 | 23 kDa |
| GenBank蛋白编号 | BC017265 |
| 基因名称 | RAB9A |
| Gene ID (NCBI) | 9367 |
| RRID | AB_3671688 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Protein A purfication |
| UNIPROT ID | P51151 |
| 储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol , pH 7.3 |
| 储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
RAB9A belongs to the small GTPase superfamily, the members of which play an important role in regulating vesicular trafficking and reside in specific intracellular compartments. RAB9A has been localized to components of the endocytic/exocytic pathway and has been implicated in recycling of membrane receptors, such as mannose 6-phosphate receptor, from early endosomes to the trans Golgi network.
实验方案
| Product Specific Protocols | |
|---|---|
| WB protocol for RAB9A antibody 84129-5-RR | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |