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RABEP1 Monoclonal antibody

RABEP1 Monoclonal Antibody for IF/ICC, WB, ELISA

Host / Isotype

Mouse / IgG1

Reactivity

Human, Mouse, Rat, Rabbit, Pig

Applications

IF/ICC, WB, ELISA

Conjugate

Unconjugated

CloneNo.

1E7E11

Cat No : 68285-1-Ig

Print datasheet

Synonyms

RAB5EP, Rabaptin 4, Rabaptin 5, Rabaptin 5alpha, RABEP1, RABPT5, RABPT5A

验证数据展示

  • WB analysis using 68285-1-Ig

    WB analysis using 68285-1-Ig

    Various lysates were subjected to SDS PAGE followed by western blot with 68285-1-Ig (RABEP1 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours.

  • IF Staining of HeLa using 68285-1-Ig

    IF Staining of HeLa using 68285-1-Ig

    Immunofluorescent analysis of (-20°C Methanol) fixed HeLa cells using RABEP1 antibody (68285-1-Ig, Clone: 1E7E11 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L).

  • IF Staining of HeLa using 68285-1-Ig

    IF Staining of HeLa using 68285-1-Ig

    Immunofluorescent analysis of (-20°C Methanol) fixed HeLa cells using RABEP1 antibody (68285-1-Ig, Clone: 1E7E11 ) at dilution of 1:800 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L).

经过测试的应用

Positive WB detected inpig brain tissue, pig cerebellum tissue, rabbit brain tissue, rabbit cerebellum tissue, rat brain tissue, rat cerebellum tissue, mouse brain tissue, mouse cerebellum tissue
Positive IF detected inHeLa cells
Planning an IF experiment? We recommend our CoraLite® Plus 488 conjugated versions of this antibody.

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:5000-1:50000
Immunofluorescence (IF)IF : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

68285-1-Ig targets RABEP1 in WB, IF, ELISA applications and shows reactivity with Human, Mouse, Rat, Rabbit, Pig samples.

Tested Applications IF/ICC, WB, ELISA Application Description
Tested Reactivity Human, Mouse, Rat, Rabbit, Pig
Immunogen RABEP1 fusion protein Ag6023 种属同源性预测
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Full Name rabaptin, RAB GTPase binding effector protein 1
Synonyms RAB5EP, Rabaptin 4, Rabaptin 5, Rabaptin 5alpha, RABEP1, RABPT5, RABPT5A
Calculated Molecular Weight 99 kDa
Observed Molecular Weight100-105 kDa
GenBank Accession NumberBC041700
Gene Symbol RABEP1
Gene ID (NCBI) 9135
Conjugate Unconjugated
Form Liquid
Purification MethodProtein G purification
UNIPROT IDQ15276
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

Rab GTPase-binding effector protein 1 is an enzyme that in humans is encoded by the RABEP1 gene. It belongs to rabaptin protein family. RABEP1 act as linker between gamma-adaptin, RAB4A and RAB5A. It is involved in endocytic membrane fusion and membrane trafficking of recycling endosomes. (PMID: 22841712)

实验方案

Product Specific Protocols
WB protocol for RABEP1 antibody 68285-1-IgDownload protocol
IF protocol for RABEP1 antibody 68285-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols