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CoraLite® Plus 488-conjugated RABEP1 Monoclonal antibody

RABEP1 Monoclonal Antibody for IF/ICC

Host / Isotype

Mouse / IgG1

Reactivity

Human, Mouse, Rat, Rabbit, Pig

Applications

IF/ICC

Conjugate

CoraLite® Plus 488 Fluorescent Dye

CloneNo.

1E7E11

Cat no : CL488-68285

Print datasheet

Synonyms

RAB5EP, Rabaptin 4, Rabaptin 5, Rabaptin 5alpha, RABEP1, RABPT5, RABPT5A

验证数据展示

  • IF Staining of HeLa using CL488-68285

    IF Staining of HeLa using CL488-68285

    Immunofluorescent analysis of (-20°C Methanol) fixed HeLa cells using CoraLite® Plus 488 RABEP1 antibody (CL488-68285, Clone: 1E7E11 ) at dilution of 1:100.

经过测试的应用

Positive IF/ICC detected inHeLa cells
For other applications, we recommend the unconjugated version of this antibody, 68285-1-Ig

推荐稀释比

ApplicationDilution
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

CL488-68285 targets RABEP1 in IF/ICC applications and shows reactivity with Human, Mouse, Rat, Rabbit, Pig samples.

Tested Applications IF/ICC Application Description
Tested Reactivity Human, Mouse, Rat, Rabbit, Pig
Immunogen RABEP1 fusion protein Ag6023 种属同源性预测
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Full Name rabaptin, RAB GTPase binding effector protein 1
Synonyms RAB5EP, Rabaptin 4, Rabaptin 5, Rabaptin 5alpha, RABEP1, RABPT5, RABPT5A
Calculated Molecular Weight 99 kDa
Observed Molecular Weight100-105 kDa
GenBank Accession NumberBC041700
Gene Symbol RABEP1
Gene ID (NCBI) 9135
Conjugate CoraLite® Plus 488 Fluorescent Dye
Excitation/Emission Maxima Wavelengths493 nm / 522 nm
Form Liquid
Purification MethodProtein G purification
UNIPROT IDQ15276
Storage Buffer PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
Storage ConditionsStore at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

Rab GTPase-binding effector protein 1 is an enzyme that in humans is encoded by the RABEP1 gene. It belongs to rabaptin protein family. RABEP1 act as linker between gamma-adaptin, RAB4A and RAB5A. It is involved in endocytic membrane fusion and membrane trafficking of recycling endosomes. (PMID: 22841712)

实验方案

Product Specific Protocols
IF protocol for CL Plus 488 RABEP1 antibody CL488-68285Download protocol
Standard Protocols
Click here to view our Standard Protocols