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验证数据展示

  • IF Staining of A549 using CL488-66622

    IF Staining of A549 using CL488-66622

    Immunofluorescent analysis of (-20°C Methanol) fixed A549 cells using CoraLite® Plus 488 RABEPK/p40 antibody (CL488-66622, Clone: 1E11A3 ) at dilution of 1:200.

CoraLite® Plus 488-conjugated RABEPK/p40 Monoclonal antibody

RABEPK/p40 Monoclonal Antibody for IF/ICC
Cat No. CL488-66622

产品说明书

CloneNo. 1E11A3

宿主/亚型

Mouse / IgG1

种属反应性

Human, mouse, rat

应用

IF/ICC

别名

40 kDa Rab9 effector protein, bA65N13.1, DKFZp686P1077, p40, RAB9P40, RABEPK

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  CoraLite® Plus 488
规格: 

-/ -

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经过测试的应用

Positive IF/ICC detected inA549 cells
Planning an IHC experiment? We recommend our IHCeasy RABEPK Ready-To-Use IHC Kit. RABEPK primary antibody included.
For other applications, we recommend the unconjugated version of this antibody, 66622-1-Ig

推荐稀释比

应用推荐稀释比
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

CL488-66622 targets RABEPK/p40 in IF/ICC applications and shows reactivity with Human, mouse, rat samples.

经测试应用 IF/ICC Application Description
经测试反应性 Human, mouse, rat
免疫原 RABEPK/p40 fusion protein Ag7796 种属同源性预测
宿主/亚型 Mouse / IgG1
抗体类别 Monoclonal
产品类型 Antibody
全称 Rab9 effector protein with kelch motifs
别名 40 kDa Rab9 effector protein, bA65N13.1, DKFZp686P1077, p40, RAB9P40, RABEPK
计算分子量 41 kDa
观测分子量 40 kDa
GenBank蛋白编号BC065725
基因名称 RABEPK/p40
Gene ID (NCBI) 10244
RRIDAB_3672918
偶联类型 CoraLite® Plus 488 Fluorescent Dye
最大激发/发射波长493 nm / 522 nm
形式 Liquid
纯化方式Protein G purification
UNIPROT IDQ7Z6M1
储存缓冲液 PBS with 50% glycerol, 0.05% Proclin300, 0.5% BSA , pH 7.3
储存条件Store at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

Rab9 GTPase is required for the transport of mannose 6-phosphate receptors from endosomes to the trans-Golgi network in living cells, and in an in vitro system that reconstitutes this process. P40 is an effector of Rab9 that interacts preferentially with the active form of Rab9. p40 does not interact with Rab7 or K-Ras; it also fails to bind Rab9 when it is bound to GDI. The protein is found in cytosol, yet a significant fraction (~30%) is associated with cellular membranes. P40 is a very potent transport factor in that the pure, recombinant protein can stimulate, significantly, an in vitro transport assay that measures transport of mannose 6-phosphate receptors from endosomes to the trans-Golgi network.

实验方案

Product Specific Protocols
IF protocol for CL Plus 488 RABEPK/p40 antibody CL488-66622Download protocol
Standard Protocols
Click here to view our Standard Protocols