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RABIF Polyclonal antibody, PBS Only

RABIF Polyclonal Antibody for WB, IHC, IF/ICC, Indirect ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IHC, IF/ICC, Indirect ELISA

Conjugate

Unconjugated

Cat no : 29113-1-PBS

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Synonyms

验证数据展示

  • IF Staining of MCF-7 using 29113-1-AP (same clone as 29113-1-PBS)

    IF Staining of MCF-7 using 29113-1-AP (same clone as 29113-1-PBS)

    Immunofluorescent analysis of (-20°C Methanol) fixed MCF-7 cells using RABIF antibody (29113-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L). This data was developed using the same antibody clone with 29113-1-PBS in a different storage buffer formulation.

  • IHC staining of mouse brain using 29113-1-AP (same clone as 29113-1-PBS)

    IHC staining of mouse brain using 29113-1-AP (same clone as 29113-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 29113-1-AP (RABIF antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 29113-1-PBS in a different storage buffer formulation.

  • WB analysis using 29113-1-AP (same clone as 29113-1-PBS)

    WB analysis using 29113-1-AP (same clone as 29113-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 29113-1-AP (RABIF antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 29113-1-PBS in a different storage buffer formulation.

产品信息

29113-1-PBS targets RABIF in WB, IHC, IF/ICC, Indirect ELISA applications and shows reactivity with human, mouse samples.

Tested Applications WB, IHC, IF/ICC, Indirect ELISA Application Description
Tested Reactivity human, mouse
Immunogen RABIF fusion protein Ag30761 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name RAB interacting factor
Synonyms
Calculated Molecular Weight 123 aa, 14 kDa
Observed Molecular Weight14 kDa
GenBank Accession NumberBC018488
Gene Symbol RABIF
Gene ID (NCBI) 5877
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDP47224
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

RABIF (Rab-interacting factor)/MSS4 (mammalian suppressor of Sec4) was the first putative guanine nucleotide exchange factors (GEFs) isolated for Rabs, which may act as a chaperone, allowing interacting Rab proteins to be properly activated where and when they are needed in the cell (PMID: 25158853). RABIF is a 17-kDa protein that is expressed in all mammalian tissues and is in complex with nucleotide-free Rab8 GTPase and Rab10 GTPase (PMID: 28894007, 28228250).