验证数据展示
经过测试的应用
| Positive WB detected in | HEK-293 cells, mouse brain tissue, human brain tissue, human lung tissue, HUVEC cells, U-251 cells |
| Positive IP detected in | mouse lung tissue |
| Positive IHC detected in | human breast cancer tissue, human liver cancer tissue, human cervical cancer tissue, human kidney tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF/ICC detected in | A549 cells |
| Positive FC (Intra) detected in | HEK-293 cells |
Planning an IF experiment? We recommend our CoraLite® Plus 488 conjugated versions of this antibody.
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Western Blot (WB) | WB : 1:1000-1:5000 |
| Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
| Immunohistochemistry (IHC) | IHC : 1:20-1:200 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:200-1:800 |
| Flow Cytometry (FC) (INTRA) | FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
发表文章中的应用
| KD/KO | See 2 publications below |
| WB | See 2 publications below |
产品信息
11916-1-AP targets RABL3 in WB, IHC, IF/ICC, FC (Intra), IP, ELISA applications and shows reactivity with human, mouse samples.
| 经测试应用 | WB, IHC, IF/ICC, FC (Intra), IP, ELISA Application Description |
| 文献引用应用 | WB |
| 经测试反应性 | human, mouse |
| 文献引用反应性 | human |
| 免疫原 | RABL3 fusion protein Ag2531 种属同源性预测 |
| 宿主/亚型 | Rabbit / IgG |
| 抗体类别 | Polyclonal |
| 产品类型 | Antibody |
| 全称 | RAB, member of RAS oncogene family-like 3 |
| 别名 | Rab-like protein 3, Rab like protein 3 |
| 计算分子量 | 236 aa, 26 kDa |
| 观测分子量 | 26 kDa |
| GenBank蛋白编号 | BC020832 |
| 基因名称 | RABL3 |
| Gene ID (NCBI) | 285282 |
| RRID | AB_2175960 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Antigen affinity purification |
| UNIPROT ID | Q5HYI8 |
| 储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol , pH 7.3 |
| 储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
The gene of Rabl3 is located on 3q13.3 and is composed of eight exons and seven introns. It is a member of the Rab subfamily, the largest group in the Ras superfamily of small guanosine triphosphatases (GTPases).
实验方案
| Product Specific Protocols | |
|---|---|
| WB protocol for RABL3 antibody 11916-1-AP | Download protocol |
| IHC protocol for RABL3 antibody 11916-1-AP | Download protocol |
| IF protocol for RABL3 antibody 11916-1-AP | Download protocol |
| IP protocol for RABL3 antibody 11916-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
发表文章
| Species | Application | Title |
|---|---|---|
Oncol Rep Evaluation of the novel gene Rabl3 in the regulation of proliferation and motility in human cancer cells.
| ||
Med Sci Monit High Expression of Rab-like 3 (Rabl3) is Associated with Poor Survival of Patients with Non-Small Cell Lung Cancer via Repression of MAPK8/9/10-Mediated Autophagy.
|
