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验证数据展示

  • IF Staining of A549 using CL488-11916

    IF Staining of A549 using CL488-11916

    Immunofluorescent analysis of (-20°C Methanol) fixed A549 cells using CoraLite® Plus 488 RABL3 antibody (CL488-11916) at dilution of 1:200.

  • FC experiment of HEK-293 using CL488-11916

    FC experiment of HEK-293 using CL488-11916

    1x10^6 HEK-293 cells were intracellularly stained with 0.8 ug CoraLite® Plus 488 Anti-Human RABL3 (CL488-11916) (red), or 0.8 ug CoraLite® Plus 488-conjugated Rabbit IgG control Rabbit PolyAb (CL488-30000, Clone: ) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

  • Featured Product
  • KD/KO验证

CoraLite® Plus 488-conjugated RABL3 Polyclonal antibody

RABL3 Polyclonal Antibody for IF/ICC, FC (Intra)
Cat No. CL488-11916

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse

应用

IF/ICC, FC (Intra)

别名

Rab like protein 3, RABL3

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  CoraLite® Plus 488
规格: 

-/ -

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经过测试的应用

Positive IF/ICC detected inA549 cells
Positive FC (Intra) detected inHEK-293 cells
Positive FC detected inHEK-293 cells
For other applications, we recommend the unconjugated version of this antibody, 11916-1-AP

推荐稀释比

应用推荐稀释比
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.80 ug per 10^6 cells in a 100 µl suspension
Flow Cytometry (FC)FC : 0.80 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

CL488-11916 targets RABL3 in IF/ICC, FC (Intra) applications and shows reactivity with human, mouse samples.

经测试应用 IF/ICC, FC (Intra) Application Description
经测试反应性 human, mouse
免疫原 RABL3 fusion protein Ag2531 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 RAB, member of RAS oncogene family-like 3
别名 Rab like protein 3, RABL3
计算分子量 236 aa, 26 kDa
观测分子量 26 kDa
GenBank蛋白编号BC020832
基因名称 RABL3
Gene ID (NCBI) 285282
RRIDAB_3672519
偶联类型 CoraLite® Plus 488 Fluorescent Dye
最大激发/发射波长493 nm / 522 nm
形式 Liquid
纯化方式Antigen affinity purification
UNIPROT IDQ5HYI8
储存缓冲液 PBS with 50% glycerol, 0.05% Proclin300, 0.5% BSA , pH 7.3
储存条件Store at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

The gene of Rabl3 is located on 3q13.3 and is composed of eight exons and seven introns. It is a member of the Rab subfamily, the largest group in the Ras superfamily of small guanosine triphosphatases (GTPases).

实验方案

Product Specific Protocols
IF protocol for CL Plus 488 RABL3 antibody CL488-11916Download protocol
Standard Protocols
Click here to view our Standard Protocols