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  • Featured Product
  • KD/KO Validated

CoraLite® Plus 488-conjugated RABL3 Polyclonal antibody

RABL3 Polyclonal Antibody for IF/ICC, FC (Intra)

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

IF/ICC, FC (Intra)

Conjugate

CoraLite® Plus 488 Fluorescent Dye

Cat no : CL488-11916

Print datasheet

Synonyms

Rab like protein 3, RABL3

验证数据展示

  • IF Staining of A549 using CL488-11916

    IF Staining of A549 using CL488-11916

    Immunofluorescent analysis of (-20°C Methanol) fixed A549 cells using CoraLite® Plus 488 RABL3 antibody (CL488-11916) at dilution of 1:200.

  • FC experiment of HEK-293 using CL488-11916

    FC experiment of HEK-293 using CL488-11916

    1x10^6 HEK-293 cells were intracellularly stained with 0.8 ug CoraLite® Plus 488 Anti-Human RABL3 (CL488-11916) (red), or 0.8 ug CoraLite® Plus 488-conjugated Rabbit IgG control Rabbit PolyAb (CL488-30000, Clone: ) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

经过测试的应用

Positive IF/ICC detected inA549 cells
Positive FC (Intra) detected inHEK-293 cells
Positive FC detected inHEK-293 cells
For other applications, we recommend the unconjugated version of this antibody, 11916-1-AP

推荐稀释比

ApplicationDilution
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.80 ug per 10^6 cells in a 100 µl suspension
Flow Cytometry (FC)FC : 0.80 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

CL488-11916 targets RABL3 in IF/ICC, FC (Intra) applications and shows reactivity with human, mouse samples.

Tested Applications IF/ICC, FC (Intra) Application Description
Tested Reactivity human, mouse
Immunogen RABL3 fusion protein Ag2531 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name RAB, member of RAS oncogene family-like 3
Synonyms Rab like protein 3, RABL3
Calculated Molecular Weight 236 aa, 26 kDa
Observed Molecular Weight 26 kDa
GenBank Accession NumberBC020832
Gene Symbol RABL3
Gene ID (NCBI) 285282
Conjugate CoraLite® Plus 488 Fluorescent Dye
Excitation/Emission Maxima Wavelengths493 nm / 522 nm
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ5HYI8
Storage Buffer PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
Storage ConditionsStore at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

The gene of Rabl3 is located on 3q13.3 and is composed of eight exons and seven introns. It is a member of the Rab subfamily, the largest group in the Ras superfamily of small guanosine triphosphatases (GTPases).

实验方案

Product Specific Protocols
IF protocol for CL Plus 488 RABL3 antibody CL488-11916Download protocol
Standard Protocols
Click here to view our Standard Protocols