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RACGAP1 Monoclonal antibody

RACGAP1 Monoclonal Antibody for WB, IHC, IF/ICC, ELISA

Host / Isotype

Mouse / IgG1

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, ELISA

Conjugate

Unconjugated

CloneNo.

6E10A2

Cat no : 66056-1-Ig

Print datasheet

Synonyms

HsCYK 4, ID GAP, KIAA1478, Male germ cell RacGap, MgcRacGAP, RACGAP1

验证数据展示

  • WB analysis of HEK-293 using 66056-1-Ig

    WB analysis of HEK-293 using 66056-1-Ig

    HEK-293, Jurkat, ROS1728, PC-12, Raw264.7, NIH/3T3 cells were subjected to SDS PAGE followed by western blot with 66056-1-Ig (RACGAP1 Antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

  • WB analysis of HEK293 cells using 66056-1-Ig

    WB analysis of HEK293 cells using 66056-1-Ig

    WB result of RACGAP1 antibody (66056-1-Ig, 1:3000) with si-control and si-RACGAP1 transfected HEK293 cells.

  • IHC staining of human colon cancer using 66056-1-Ig

    IHC staining of human colon cancer using 66056-1-Ig

    Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 66056-1-Ig (RACGAP1 Antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IHC staining of human colon cancer using 66056-1-Ig

    IHC staining of human colon cancer using 66056-1-Ig

    Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 66056-1-Ig (RACGAP1 Antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IF Staining of HeLa using 66056-1-Ig

    IF Staining of HeLa using 66056-1-Ig

    Immunofluorescent analysis of HeLa cells, using RACGAP1 antibody 66056-1-lg at 1:50 dilution and Rhodamine-labeled goat anti-mouse IgG (red).

经过测试的应用

Positive WB detected inHEK-293 cells, HEK293 cells
Positive IHC detected inhuman colon cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inHela cells
Planning an IF experiment? We recommend our CoraLite® Plus 488 and CoraLite®594 conjugated versions of this antibody.

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:4000
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)/ICCIF/ICC : 1:20-1:200
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

The immunogen of 66056-1-Ig is RACGAP1 Fusion Protein expressed in E. coli.

Tested Applications WB, IHC, IF/ICC, ELISA Application Description
Tested Reactivity human, mouse, rat
Immunogen RACGAP1 fusion protein Ag6944 种属同源性预测
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Full Name Rac GTPase activating protein 1
Synonyms HsCYK 4, ID GAP, KIAA1478, Male germ cell RacGap, MgcRacGAP, RACGAP1
Calculated Molecular Weight 632 aa, 71 kDa
Observed Molecular Weight 71 kDa
GenBank Accession NumberBC032754
Gene Symbol RACGAP1
Gene ID (NCBI) 29127
RRIDAB_11043178
Conjugate Unconjugated
Form Liquid
Purification MethodProtein G purification
UNIPROT IDQ9H0H5
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

Rac GTPase-activating protein 1 (RACGAP1), localized in the mitotic spindle, has guanosine triphosphatase-activating protein (GAP) activity for the Rho family GTPases. RACGAP1 has an important influence on the initiation of cytokinesis, control of cell growth and differentiation, regulation of spermatogenesis, and tumor metastasis.

实验方案

Product Specific Protocols
WB protocol for RACGAP1 antibody 66056-1-IgDownload protocol
IHC protocol for RACGAP1 antibody 66056-1-IgDownload protocol
IF protocol for RACGAP1 antibody 66056-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols