- Featured Product
- KD/KO Validated
CoraLite® Plus 488-conjugated RACGAP1 Monoclonal antibody
RACGAP1 Monoclonal Antibody for IF/ICC
Host / Isotype
Mouse / IgG1
Reactivity
human, mouse, rat
Applications
IF/ICC
Conjugate
CoraLite® Plus 488 Fluorescent Dye
CloneNo.
6E10A2
验证数据展示
经过测试的应用
Positive IF/ICC detected in | HeLa cells |
For other applications, we recommend the unconjugated version of this antibody, 66056-1-Ig
推荐稀释比
Application | Dilution |
---|---|
Immunofluorescence (IF)/ICC | IF/ICC : 1:50-1:500 |
It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
Sample-dependent, Check data in validation data gallery. |
产品信息
The immunogen of CL488-66056 is RACGAP1 Fusion Protein expressed in E. coli.
Tested Applications | IF/ICC Application Description |
Tested Reactivity | human, mouse, rat |
Immunogen | RACGAP1 fusion protein Ag6944 种属同源性预测 |
Host / Isotype | Mouse / IgG1 |
Class | Monoclonal |
Type | Antibody |
Full Name | Rac GTPase activating protein 1 |
Synonyms | HsCYK 4, ID GAP, KIAA1478, Male germ cell RacGap, MgcRacGAP, RACGAP1 |
Calculated Molecular Weight | 632 aa, 71 kDa |
Observed Molecular Weight | 71 kDa |
GenBank Accession Number | BC032754 |
Gene Symbol | RACGAP1 |
Gene ID (NCBI) | 29127 |
RRID | AB_2883243 |
Conjugate | CoraLite® Plus 488 Fluorescent Dye |
Excitation/Emission Maxima Wavelengths | 493 nm / 522 nm |
Form | Liquid |
Purification Method | Protein G purification |
UNIPROT ID | Q9H0H5 |
Storage Buffer | PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3. |
Storage Conditions | Store at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
Rac GTPase-activating protein 1 (RACGAP1), localized in the mitotic spindle, has guanosine triphosphatase-activating protein (GAP) activity for the Rho family GTPases. RACGAP1 has an important influence on the initiation of cytokinesis, control of cell growth and differentiation, regulation of spermatogenesis, and tumor metastasis.
实验方案
Product Specific Protocols | |
---|---|
IF protocol for CL Plus 488 RACGAP1 antibody CL488-66056 | Download protocol |
Standard Protocols | |
---|---|
Click here to view our Standard Protocols |