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验证数据展示

  • IF Staining of HeLa using CL488-66056

    IF Staining of HeLa using CL488-66056

    Immunofluorescent analysis of (4% PFA) fixed HeLa cells using CL488-66056 (RACGAP1 antibody) at dilution of 1:100.

  • Featured Product
  • KD/KO Validated

CoraLite® Plus 488-conjugated RACGAP1 Monoclonal antibody

RACGAP1 Monoclonal Antibody for IF/ICC
Cat No. CL488-66056

Print datasheet

CloneNo. 6E10A2

Host / Isotype

Mouse / IgG1

Reactivity

human, mouse, rat

Applications

IF/ICC

Synonyms

HsCYK 4, ID GAP, KIAA1478, Male germ cell RacGap, MgcRacGAP, RACGAP1

Formulation:  PBS and Azide
PBS and Azide
Conjugate:  CoraLite® Plus 488
Size: 

-/ -

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经过测试的应用

Positive IF/ICC detected inHeLa cells
For other applications, we recommend the unconjugated version of this antibody, 66056-1-Ig

推荐稀释比

ApplicationDilution
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

CL488-66056 targets RACGAP1 in IF/ICC applications and shows reactivity with human, mouse, rat samples.

Tested Applications IF/ICC Application Description
Tested Reactivity human, mouse, rat
Immunogen RACGAP1 fusion protein Ag6944 种属同源性预测
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Full Name Rac GTPase activating protein 1
Synonyms HsCYK 4, ID GAP, KIAA1478, Male germ cell RacGap, MgcRacGAP, RACGAP1
Calculated Molecular Weight 632 aa, 71 kDa
Observed Molecular Weight 71 kDa
GenBank Accession NumberBC032754
Gene Symbol RACGAP1
Gene ID (NCBI) 29127
RRIDAB_2883243
Conjugate CoraLite® Plus 488 Fluorescent Dye
Excitation/Emission Maxima Wavelengths493 nm / 522 nm
Form Liquid
Purification MethodProtein G purification
UNIPROT IDQ9H0H5
Storage Buffer PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
Storage ConditionsStore at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

Rac GTPase-activating protein 1 (RACGAP1), localized in the mitotic spindle, has guanosine triphosphatase-activating protein (GAP) activity for the Rho family GTPases. RACGAP1 has an important influence on the initiation of cytokinesis, control of cell growth and differentiation, regulation of spermatogenesis, and tumor metastasis.

实验方案

Product Specific Protocols
IF protocol for CL Plus 488 RACGAP1 antibody CL488-66056Download protocol
Standard Protocols
Click here to view our Standard Protocols