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RACGAP1 Recombinant antibody, PBS Only

RACGAP1 Uni-rAbTM Recombinant Antibody for WB, IHC, IF/ICC, FC (Intra), Indirect ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IHC, IF/ICC, FC (Intra), Indirect ELISA

Conjugate

Unconjugated

CloneNo.

2D5

Cat no : 82788-1-PBS

Print datasheet

Synonyms

HsCYK4, HsCYK 4, Gap1, GAP, CYK4

验证数据展示

  • WB analysis using 82788-1-RR (same clone as 82788-1-PBS)

    WB analysis using 82788-1-RR (same clone as 82788-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 82788-1-RR (RACGAP1 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 82788-1-PBS in a different storage buffer formulation.

  • FC experiment of HeLa using 82788-1-RR (same clone as 82788-1-PBS)

    FC experiment of HeLa using 82788-1-RR (same clone as 82788-1-PBS)

    1x10^6 HeLa cells were intracellularly stained with 0.25 ug RACGAP1 Recombinant antibody (82788-1-RR, Clone:2D5) and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2)(red), or 0.25 ug Isotype Control (blue). Cells were fixed and permeabilized with Transcription Factor Staining Buffer Kit (PF00011). This data was developed using the same antibody clone with 82788-1-PBS in a different storage buffer formulation.

  • IHC staining of human skin cancer using 82788-1-RR (same clone as 82788-1-PBS)

    IHC staining of human skin cancer using 82788-1-RR (same clone as 82788-1-PBS)

    Immunohistochemical analysis of paraffin-embedded skin cancer slide using 82788-1-RR (RACGAP1 antibody) at dilution of 1:1000 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 82788-1-PBS in a different storage buffer formulation.

  • IF Staining of U2OS using 82788-1-RR (same clone as 82788-1-PBS)

    IF Staining of U2OS using 82788-1-RR (same clone as 82788-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed U2OS cells using RACGAP1 antibody (82788-1-RR, Clone: 2D5 ) at dilution of 1:1000 and CoraLite®594-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L). This data was developed using the same antibody clone with 82788-1-PBS in a different storage buffer formulation.

  • Affinity and Kinetic Characterization of 82788-1-RR

    Affinity and Kinetic Characterization of 82788-1-RR

    Biolayer interferometry (BLl) kinetic assays of 82788-1-RR against Human RACGAP1 were performed. The affinity constant is 0.164 nM.

产品信息

82788-1-PBS targets RACGAP1 in WB, IHC, IF/ICC, FC (Intra), Indirect ELISA applications and shows reactivity with human samples.

Tested Applications WB, IHC, IF/ICC, FC (Intra), Indirect ELISA Application Description
Tested Reactivity human
Immunogen RACGAP1 fusion protein Ag4676 种属同源性预测
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Full Name Rac GTPase activating protein 1
Synonyms HsCYK4, HsCYK 4, Gap1, GAP, CYK4
Calculated Molecular Weight 632 aa, 71 kDa
Observed Molecular Weight 71 kDa
GenBank Accession NumberBC032754
Gene Symbol RACGAP1
Gene ID (NCBI) 29127
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
UNIPROT IDQ9H0H5
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

Rac GTPase-activating protein 1 (RACGAP1), localized in the mitotic spindle, has guanosine triphosphatase-activating protein (GAP) activity for the Rho family GTPases. RACGAP1 has an important influence on the initiation of cytokinesis, control of cell growth and differentiation, regulation of spermatogenesis, and tumor metastasis.