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Host
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Species Reactivity
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Species Reactivity
0
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0
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Compatible Species
0
Conjugate
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验证数据展示

  • WB analysis using 82788-1-RR (same clone as 82788-1-PBS)

    WB analysis using 82788-1-RR (same clone as 82788-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 82788-1-RR (RACGAP1 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 82788-1-PBS in a different storage buffer formulation.

  • FC experiment of HeLa using 82788-1-RR (same clone as 82788-1-PBS)

    FC experiment of HeLa using 82788-1-RR (same clone as 82788-1-PBS)

    1x10^6 HeLa cells were intracellularly stained with 0.25 ug RACGAP1 Recombinant antibody (82788-1-RR, Clone:2D5) and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2)(red), or 0.25 ug Isotype Control (blue). Cells were fixed and permeabilized with Transcription Factor Staining Buffer Kit (PF00011). This data was developed using the same antibody clone with 82788-1-PBS in a different storage buffer formulation.

  • IHC staining of human skin cancer using 82788-1-RR (same clone as 82788-1-PBS)

    IHC staining of human skin cancer using 82788-1-RR (same clone as 82788-1-PBS)

    Immunohistochemical analysis of paraffin-embedded skin cancer slide using 82788-1-RR (RACGAP1 antibody) at dilution of 1:1000 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 82788-1-PBS in a different storage buffer formulation.

  • IF Staining of U2OS using 82788-1-RR (same clone as 82788-1-PBS)

    IF Staining of U2OS using 82788-1-RR (same clone as 82788-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed U2OS cells using RACGAP1 antibody (82788-1-RR, Clone: 2D5 ) at dilution of 1:1000 and CoraLite®594-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L). This data was developed using the same antibody clone with 82788-1-PBS in a different storage buffer formulation.

  • Affinity and Kinetic Characterization of 82788-1-RR

    Affinity and Kinetic Characterization of 82788-1-RR

    Biolayer interferometry (BLl) kinetic assays of 82788-1-RR against Human RACGAP1 were performed. The affinity constant is 0.164 nM.

RACGAP1 Recombinant antibody, PBS Only

RACGAP1 Uni-rAbTM Recombinant Antibody for WB, IHC, IF/ICC, FC (Intra), Indirect ELISA
Cat No. 82788-1-PBS

产品说明书

CloneNo. 2D5

宿主/亚型

Rabbit / IgG

种属反应性

human

应用

WB, IHC, IF/ICC, FC (Intra), Indirect ELISA

别名

HsCYK4, HsCYK 4, Gap1, GAP, CYK4

缓冲液配方:  PBS Only
PBS and Azide
PBS Only
偶联物:  Unconjugated
规格: 

-/ -

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产品信息

82788-1-PBS targets RACGAP1 in WB, IHC, IF/ICC, FC (Intra), Indirect ELISA applications and shows reactivity with human samples.

经测试应用 WB, IHC, IF/ICC, FC (Intra), Indirect ELISA Application Description
经测试反应性 human
免疫原 RACGAP1 fusion protein Ag4676 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Recombinant
产品类型 Antibody
全称 Rac GTPase activating protein 1
别名 HsCYK4, HsCYK 4, Gap1, GAP, CYK4
计算分子量 632 aa, 71 kDa
观测分子量 71 kDa
GenBank蛋白编号BC032754
基因名称 RACGAP1
Gene ID (NCBI) 29127
偶联类型 Unconjugated
形式 Liquid
纯化方式Protein A purification
UNIPROT IDQ9H0H5
储存缓冲液 PBS only , pH 7.3
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

Rac GTPase-activating protein 1 (RACGAP1), localized in the mitotic spindle, has guanosine triphosphatase-activating protein (GAP) activity for the Rho family GTPases. RACGAP1 has an important influence on the initiation of cytokinesis, control of cell growth and differentiation, regulation of spermatogenesis, and tumor metastasis.