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RAD1 Monoclonal antibody

RAD1 Monoclonal Antibody for IP, WB, ELISA

Host / Isotype

Mouse / IgG2a

Reactivity

Human

Applications

IP, WB, ELISA

Conjugate

Unconjugated

CloneNo.

1A11E2

Cat No : 68637-1-Ig

Print datasheet

Synonyms

HRAD1, RAD1, RAD1 homolog (S. pombe), REC1

验证数据展示

  • WB analysis using 68637-1-Ig

    WB analysis using 68637-1-Ig

    Various lysates were subjected to SDS PAGE followed by western blot with 68637-1-Ig (RAD1 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

  • IP experiment of HeLa using 68637-1-Ig

    IP experiment of HeLa using 68637-1-Ig

    IP result of anti-RAD1 (IP:68637-1-Ig, 4ug; Detection:68637-1-Ig 1:1000) with HeLa cells lysate 1320 ug.

经过测试的应用

Positive WB detected inMKN-45 cells, U2OS cells, HeLa cells, HEK-293 cells, Jurkat cells, MOLT-4 cells, Raji cells, Daudi cells, K-562 cells
Positive IP detected inHeLa cells

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:2000-1:10000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

68637-1-Ig targets RAD1 in WB, IP, ELISA applications and shows reactivity with Human samples.

Tested Applications IP, WB, ELISA Application Description
Tested Reactivity Human
Immunogen RAD1 fusion protein Ag33257 种属同源性预测
Host / Isotype Mouse / IgG2a
Class Monoclonal
Type Antibody
Full Name RAD1 homolog (S. pombe)
Synonyms HRAD1, RAD1, RAD1 homolog (S. pombe), REC1
Calculated Molecular Weight 32 kDa
Observed Molecular Weight 32 kDa
GenBank Accession NumberBC006837
Gene Symbol RAD1
Gene ID (NCBI) 5810
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
UNIPROT IDO60671
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

RAD1 is also named as REC1,hRAD1. The human RAD1 and HUS1 proteins associate in a complex that interacts with a highly modified form of RAD9. They concluded that these 3 proteins are central components of a DNA damage-responsive protein complex in human cells.It has 3 isoforms produced by alternative splicing.

实验方案

Product Specific Protocols
WB protocol for RAD1 antibody 68637-1-IgDownload protocol
IP protocol for RAD1 antibody 68637-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols