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验证数据展示

  • WB analysis using 13358-1-AP

    WB analysis using 13358-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 13358-1-AP (RAD17 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

  • IHC staining of human breast cancer using 13358-1-AP

    IHC staining of human breast cancer using 13358-1-AP

    Immunohistochemical analysis of paraffin-embedded human breast cancer using 13358-1-AP (RAD17 antibody) at dilution of 1:100 (under 10x lens).

    Ready-to-use IHC kits available with RAD17 antibody included
  • IHC staining of human breast cancer using 13358-1-AP

    IHC staining of human breast cancer using 13358-1-AP

    Immunohistochemical analysis of paraffin-embedded human breast cancer using 13358-1-AP (RAD17 antibody) at dilution of 1:100 (under 40x lens).

    Ready-to-use IHC kits available with RAD17 antibody included
  • IF Staining of HeLa using 13358-1-AP

    IF Staining of HeLa using 13358-1-AP

    Immunofluorescent analysis of (4% PFA) fixed HeLa cells using 13358-1-AP (RAD17 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

  • FC experiment of A431 using 13358-1-AP

    FC experiment of A431 using 13358-1-AP

    1x10^6 A431 cells were intracellularly stained with 0.25 ug RAD17 Polyclonal antibody (13358-1-AP) and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2)(red), or 0.25 ug Rabbit IgG control Rabbit PolyAb (30000-0-AP) (blue). Cells were fixed and permeabilized with Transcription Factor Staining Buffer Kit (PF00011).

  • Featured Product
  • KD/KO验证

RAD17 Polyclonal antibody

RAD17 Polyclonal Antibody for WB, IHC, IF/ICC, FC (Intra), ELISA
Cat No. 13358-1-AP

产品说明书

引用文献(3)

宿主/亚型

Rabbit / IgG

种属反应性

human and More (1)

应用

WB, IHC, IF/ICC, FC (Intra), ELISA

别名

RF-C/activator 1 homolog, R24L, HRAD17

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  Unconjugated
Unconjugated
规格: 

-/ -

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经过测试的应用

Positive WB detected inK-562 cells, Raji cells
Positive IHC detected inhuman breast cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inHeLa cells
Positive FC (Intra) detected inA431 cells
Planning an IHC experiment? We recommend our IHCeasy RAD17 Ready-To-Use IHC Kit. RAD17 primary antibody included.

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:1000-1:6000
Immunohistochemistry (IHC)IHC : 1:20-1:200
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.25 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

13358-1-AP targets RAD17 in WB, IHC, IF/ICC, FC (Intra), ELISA applications and shows reactivity with human samples.

经测试应用 WB, IHC, IF/ICC, FC (Intra), ELISA Application Description
文献引用应用WB
经测试反应性 human
文献引用反应性human, mouse
免疫原 RAD17 fusion protein Ag4171 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 RAD17 homolog (S. pombe)
别名 RF-C/activator 1 homolog, R24L, HRAD17
计算分子量 681 aa, 71 kDa
观测分子量 71 kDa
GenBank蛋白编号BC032304
基因名称 RAD17
Gene ID (NCBI) 5884
RRIDAB_2269268
偶联类型 Unconjugated
形式 Liquid
纯化方式Antigen affinity purification
UNIPROT IDO75943
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

实验方案

Product Specific Protocols
WB protocol for RAD17 antibody 13358-1-APDownload protocol
IHC protocol for RAD17 antibody 13358-1-APDownload protocol
IF protocol for RAD17 antibody 13358-1-APDownload protocol
FC protocol for RAD17 antibody 13358-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
humanWB

Oncogenesis

The TIP60-ATM axis regulates replication fork stability in BRCA-deficient cells.

Authors - Emily M Schleicher
  • KD Validated
View Article
mouseWB

Biochem J

Mutation of the RelA(p65) Thr505 phosphosite disrupts the DNA replication stress response leading to CHK1 inhibitor resistance

Authors - Jill E Hunter
View Article
mouseWB

Biochem J

Regulation of CHK1 inhibitor resistance by a c-Rel and USP1 dependent pathway

Authors - Jill E Hunter
View Article