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  • Featured Product
  • KD/KO Validated

CoraLite® Plus 488-conjugated RAD21 Polyclonal antibody

RAD21 Polyclonal Antibody for IF/ICC

Host / Isotype

Rabbit / IgG

Reactivity

human, canine

Applications

IF/ICC

Conjugate

CoraLite® Plus 488 Fluorescent Dye

Cat no : CL488-27071

Print datasheet

Synonyms

FLJ25655, FLJ40596, hHR21, HR21, HRAD21, KIAA0078, MCD1, Nuclear matrix protein 1, NXP 1, NXP1, RAD21, RAD21 homolog (S. pombe), SCC1, SCC1 homolog

验证数据展示

  • IF Staining of MDCK using CL488-27071

    IF Staining of MDCK using CL488-27071

    Immunofluorescent analysis of (4% PFA) fixed MDCK cells using CoraLite® Plus 488 RAD21 antibody (CL488-27071) at dilution of 1:200.

经过测试的应用

Positive IF/ICC detected inMDCK cells
Planning an IHC experiment? We recommend our IHCeasy RAD21 Ready-To-Use IHC Kit. RAD21 primary antibody included.
For other applications, we recommend the unconjugated version of this antibody, 27071-1-AP

推荐稀释比

ApplicationDilution
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

CL488-27071 targets RAD21 in IF/ICC applications and shows reactivity with human, canine samples.

Tested Applications IF/ICC Application Description
Tested Reactivity human, canine
Immunogen RAD21 fusion protein Ag25481 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name RAD21 homolog (S. pombe)
Synonyms FLJ25655, FLJ40596, hHR21, HR21, HRAD21, KIAA0078, MCD1, Nuclear matrix protein 1, NXP 1, NXP1, RAD21, RAD21 homolog (S. pombe), SCC1, SCC1 homolog
Calculated Molecular Weight 631 aa, 72 kDa
Observed Molecular Weight 120 kDa
GenBank Accession NumberBC050381
Gene Symbol RAD21
Gene ID (NCBI) 5885
Conjugate CoraLite® Plus 488 Fluorescent Dye
Excitation/Emission Maxima Wavelengths493 nm / 522 nm
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDO60216
Storage Buffer PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
Storage ConditionsStore at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

RAD21, also named as Nuclear matrix protein 1, is a 631 amino acid protein, which belongs to the rad21 family. The calculated molecular weight of RAD21 is 72 kDa, but the phosphorylated RAD21 is about 120 kDa. The protein encoded by this gene is highly similar to the gene product of Schizosaccharomyces pombe rad21, a gene involved in the repair of DNA double-strand breaks, as well as in chromatid cohesion during mitosis. This protein is a nuclear phospho-protein, which becomes hyperphosphorylated in cell cycle M phase. The highly regulated association of this protein with mitotic chromatin specifically at the centromere region suggests its role in sister chromatid cohesion in mitotic cells

实验方案

Product Specific Protocols
IF protocol for CL Plus 488 RAD21 antibody CL488-27071Download protocol
Standard Protocols
Click here to view our Standard Protocols