验证数据展示
经过测试的应用
| Positive IF/ICC detected in | MDCK cells |
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Immunofluorescence (IF)/ICC | IF/ICC : 1:50-1:500 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
CL488-27071 targets RAD21 in IF/ICC applications and shows reactivity with human, canine samples.
| 经测试应用 | IF/ICC Application Description |
| 经测试反应性 | human, canine |
| 免疫原 | RAD21 fusion protein Ag25481 种属同源性预测 |
| 宿主/亚型 | Rabbit / IgG |
| 抗体类别 | Polyclonal |
| 产品类型 | Antibody |
| 全称 | RAD21 homolog (S. pombe) |
| 别名 | HR21, hHR21, FLJ40596, FLJ25655, Double-strand-break repair protein rad21 homolog |
| 计算分子量 | 631 aa, 72 kDa |
| 观测分子量 | 120 kDa |
| GenBank蛋白编号 | BC050381 |
| 基因名称 | RAD21 |
| Gene ID (NCBI) | 5885 |
| RRID | AB_3084105 |
| 偶联类型 | CoraLite® Plus 488 Fluorescent Dye |
| 最大激发/发射波长 | 493 nm / 522 nm |
| 形式 | Liquid |
| 纯化方式 | Antigen affinity purification |
| UNIPROT ID | O60216 |
| 储存缓冲液 | PBS with 50% glycerol, 0.05% Proclin300, 0.5% BSA , pH 7.3 |
| 储存条件 | Store at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
RAD21, also named as Nuclear matrix protein 1, is a 631 amino acid protein, which belongs to the rad21 family. The calculated molecular weight of RAD21 is 72 kDa, but the phosphorylated RAD21 is about 120 kDa. The protein encoded by this gene is highly similar to the gene product of Schizosaccharomyces pombe rad21, a gene involved in the repair of DNA double-strand breaks, as well as in chromatid cohesion during mitosis. This protein is a nuclear phospho-protein, which becomes hyperphosphorylated in cell cycle M phase. The highly regulated association of this protein with mitotic chromatin specifically at the centromere region suggests its role in sister chromatid cohesion in mitotic cells
实验方案
| Product Specific Protocols | |
|---|---|
| IF protocol for CL Plus 488 RAD21 antibody CL488-27071 | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |