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RAD23B Monoclonal antibody, PBS Only

RAD23B Monoclonal Antibody for WB, IHC, IF/ICC, Indirect ELISA

Host / Isotype

Mouse / IgG2a

Reactivity

Human, Mouse, Rat, Pig

Applications

WB, IHC, IF/ICC, Indirect ELISA

Conjugate

Unconjugated

CloneNo.

2E6G9

Cat no : 67988-1-PBS

Print datasheet

Synonyms

HHR23B, HR23B, P58, RAD23B

验证数据展示

  • WB analysis using 67988-1-Ig (same clone as 67988-1-PBS)

    WB analysis using 67988-1-Ig (same clone as 67988-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 67988-1-Ig (RAD23B antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67988-1-PBS in a different storage buffer formulation.

  • WB analysis using 67988-1-Ig (same clone as 67988-1-PBS)

    WB analysis using 67988-1-Ig (same clone as 67988-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 67988-1-Ig (RAD23B antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67988-1-PBS in a different storage buffer formulation.

  • IHC staining of mouse brain using 67988-1-Ig (same clone as 67988-1-PBS)

    IHC staining of mouse brain using 67988-1-Ig (same clone as 67988-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 67988-1-Ig (RAD23B antibody) at dilution of 1:2000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67988-1-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with RAD23B antibody included
  • IF Staining of HeLa using 67988-1-Ig (same clone as 67988-1-PBS)

    IF Staining of HeLa using 67988-1-Ig (same clone as 67988-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed HeLa cells using RAD23B antibody (67988-1-Ig, Clone: 2E6G9 ) at dilution of 1:800 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L), CL594-Phalloidin (red). This data was developed using the same antibody clone with 67988-1-PBS in a different storage buffer formulation.

Planning an IHC experiment? We recommend our IHCeasy RAD23B Ready-To-Use IHC Kit. RAD23B primary antibody included.

产品信息

67988-1-PBS targets RAD23B in WB, IHC, IF/ICC, Indirect ELISA applications and shows reactivity with Human, Mouse, Rat, Pig samples.

Tested Applications WB, IHC, IF/ICC, Indirect ELISA Application Description
Tested Reactivity Human, Mouse, Rat, Pig
Immunogen RAD23B fusion protein Ag28658 种属同源性预测
Host / Isotype Mouse / IgG2a
Class Monoclonal
Type Antibody
Full Name RAD23 homolog B (S. cerevisiae)
Synonyms HHR23B, HR23B, P58, RAD23B
Calculated Molecular Weight 409 aa, 43 kDa
Observed Molecular Weight58 kDa
GenBank Accession NumberBC020973
Gene Symbol RAD23B
Gene ID (NCBI) 5887
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
UNIPROT IDP54727
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

RAD23B, also named as p58, hHR23B and HR23B, belongs to the RAD23 family. It plays a central role both in proteosomal degradation of misfolded proteins and DNA repair. RAD23B is a central component of a complex required to couple deglycosylation and proteasome-mediated degradation of misfolded proteins in the endoplasmic reticulun that are retrotranslocated in the cytosol. Involved in DNA excision repair by stabilizing XPC protein. It may play a part in DNA damage recognition and/or in altering chromatin structure to allow access by damage-processing enzymes. RAD23B has two subunit: Heterodimer of a 125 kDa subunit (p125) and of a 58 kDa subunit (p58).