验证数据展示
经过测试的应用
Positive WB detected in | LNCaP cells, pig brain tissue, Hela cells, HEK-293 cells, Huh-7 cells, human platelet cells, rat brain tissue, mouse brain tissue |
Positive IHC detected in | mouse brain tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
Positive IF/ICC detected in | HeLa cells |
推荐稀释比
应用 | 推荐稀释比 |
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Western Blot (WB) | WB : 1:5000-1:50000 |
Immunohistochemistry (IHC) | IHC : 1:1000-1:4000 |
Immunofluorescence (IF)/ICC | IF/ICC : 1:400-1:1600 |
It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
Sample-dependent, Check data in validation data gallery. |
产品信息
67988-1-Ig targets RAD23B in WB, IHC, IF/ICC, ELISA applications and shows reactivity with Human, Mouse, Rat, Pig samples.
经测试应用 | WB, IHC, IF/ICC, ELISA Application Description |
经测试反应性 | Human, Mouse, Rat, Pig |
免疫原 | RAD23B fusion protein Ag28658 种属同源性预测 |
宿主/亚型 | Mouse / IgG2a |
抗体类别 | Monoclonal |
产品类型 | Antibody |
全称 | RAD23 homolog B (S. cerevisiae) |
别名 | HHR23B, HR23B, P58, RAD23B |
计算分子量 | 409 aa, 43 kDa |
观测分子量 | 58 kDa |
GenBank蛋白编号 | BC020973 |
基因名称 | RAD23B |
Gene ID (NCBI) | 5887 |
RRID | AB_2918737 |
偶联类型 | Unconjugated |
形式 | Liquid |
纯化方式 | Protein A purification |
UNIPROT ID | P54727 |
储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol , pH 7.3 |
储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
RAD23B, also named as p58, hHR23B and HR23B, belongs to the RAD23 family. It plays a central role both in proteosomal degradation of misfolded proteins and DNA repair. RAD23B is a central component of a complex required to couple deglycosylation and proteasome-mediated degradation of misfolded proteins in the endoplasmic reticulun that are retrotranslocated in the cytosol. Involved in DNA excision repair by stabilizing XPC protein. It may play a part in DNA damage recognition and/or in altering chromatin structure to allow access by damage-processing enzymes. RAD23B has two subunit: Heterodimer of a 125 kDa subunit (p125) and of a 58 kDa subunit (p58).
实验方案
Product Specific Protocols | |
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WB protocol for RAD23B antibody 67988-1-Ig | Download protocol |
IHC protocol for RAD23B antibody 67988-1-Ig | Download protocol |
IF protocol for RAD23B antibody 67988-1-Ig | Download protocol |
Standard Protocols | |
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Click here to view our Standard Protocols |