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RAD23B Monoclonal antibody

RAD23B Monoclonal Antibody for WB, IHC, IF/ICC, ELISA

Host / Isotype

Mouse / IgG2a

Reactivity

Human, Mouse, Rat, Pig

Applications

WB, IHC, IF/ICC, ELISA

Conjugate

Unconjugated

CloneNo.

2E6G9

Cat no : 67988-1-Ig

Print datasheet

Synonyms

HHR23B, HR23B, P58, RAD23B

验证数据展示

  • WB analysis using 67988-1-Ig

    WB analysis using 67988-1-Ig

    Various lysates were subjected to SDS PAGE followed by western blot with 67988-1-Ig (RAD23B antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours.

  • WB analysis using 67988-1-Ig

    WB analysis using 67988-1-Ig

    Various lysates were subjected to SDS PAGE followed by western blot with 67988-1-Ig (RAD23B antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours.

  • IHC staining of mouse brain using 67988-1-Ig

    IHC staining of mouse brain using 67988-1-Ig

    Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 67988-1-Ig (RAD23B antibody) at dilution of 1:2000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with RAD23B antibody included
  • IF Staining of HeLa using 67988-1-Ig

    IF Staining of HeLa using 67988-1-Ig

    Immunofluorescent analysis of (4% PFA) fixed HeLa cells using RAD23B antibody (67988-1-Ig, Clone: 2E6G9 ) at dilution of 1:800 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L), CL594-Phalloidin (red).

经过测试的应用

Positive WB detected inLNCaP cells, pig brain tissue, Hela cells, HEK-293 cells, Huh-7 cells, human platelet cells, rat brain tissue, mouse brain tissue
Positive IHC detected inmouse brain tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inHeLa cells
Planning an IHC experiment? We recommend our IHCeasy RAD23B Ready-To-Use IHC Kit. RAD23B primary antibody included.
Planning an IF experiment? We recommend our CoraLite® Plus 488 conjugated versions of this antibody.

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:5000-1:50000
Immunohistochemistry (IHC)IHC : 1:1000-1:4000
Immunofluorescence (IF)/ICCIF/ICC : 1:400-1:1600
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

67988-1-Ig targets RAD23B in WB, IHC, IF/ICC, ELISA applications and shows reactivity with Human, Mouse, Rat, Pig samples.

Tested Applications WB, IHC, IF/ICC, ELISA Application Description
Tested Reactivity Human, Mouse, Rat, Pig
Immunogen RAD23B fusion protein Ag28658 种属同源性预测
Host / Isotype Mouse / IgG2a
Class Monoclonal
Type Antibody
Full Name RAD23 homolog B (S. cerevisiae)
Synonyms HHR23B, HR23B, P58, RAD23B
Calculated Molecular Weight 409 aa, 43 kDa
Observed Molecular Weight58 kDa
GenBank Accession NumberBC020973
Gene Symbol RAD23B
Gene ID (NCBI) 5887
RRIDAB_2918737
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
UNIPROT IDP54727
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

RAD23B, also named as p58, hHR23B and HR23B, belongs to the RAD23 family. It plays a central role both in proteosomal degradation of misfolded proteins and DNA repair. RAD23B is a central component of a complex required to couple deglycosylation and proteasome-mediated degradation of misfolded proteins in the endoplasmic reticulun that are retrotranslocated in the cytosol. Involved in DNA excision repair by stabilizing XPC protein. It may play a part in DNA damage recognition and/or in altering chromatin structure to allow access by damage-processing enzymes. RAD23B has two subunit: Heterodimer of a 125 kDa subunit (p125) and of a 58 kDa subunit (p58).

实验方案

Product Specific Protocols
WB protocol for RAD23B antibody 67988-1-IgDownload protocol
IHC protocol for RAD23B antibody 67988-1-IgDownload protocol
IF protocol for RAD23B antibody 67988-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols