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CoraLite® Plus 488-conjugated RAD23B Monoclonal antibody

RAD23B Monoclonal Antibody for IF/ICC, FC (Intra)

Host / Isotype

Mouse / IgG2a

Reactivity

human, mouse, rat, pig

Applications

IF/ICC, FC (Intra)

Conjugate

CoraLite® Plus 488 Fluorescent Dye

CloneNo.

2E6G9

Cat no : CL488-67988

Print datasheet

Synonyms

XP-C repair-complementing complex 58 kDa protein, p58, HR23B, HHR23B, 2E6G9

验证数据展示

  • FC experiment of HeLa using CL488-67988

    FC experiment of HeLa using CL488-67988

    1X10^6 HeLa cells were intracellularly stained with 0.4 ug CoraLite® Plus 488 Anti-Human RAD23B (CL488-67988, Clone:2E6G9) (red), or 0.4 ug Mouse IgG2a Isotype Control (CL488-66360-2, Clone: K11A1B2A2) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

  • IF Staining of HeLa using CL488-67988

    IF Staining of HeLa using CL488-67988

    Immunofluorescent analysis of (4% PFA) fixed HeLa cells using CoraLite® Plus 488 RAD23B antibody (CL488-67988, Clone: 2E6G9 ) at dilution of 1:200, CL594-Phalloidin (red).

经过测试的应用

Positive IF/ICC detected inHeLa cells
Positive FC (Intra) detected inHeLa cells
Planning an IHC experiment? We recommend our IHCeasy RAD23B Ready-To-Use IHC Kit. RAD23B primary antibody included.
For other applications, we recommend the unconjugated version of this antibody, 67988-1-Ig

推荐稀释比

ApplicationDilution
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

CL488-67988 targets RAD23B in IF/ICC, FC (Intra) applications and shows reactivity with human, mouse, rat, pig samples.

Tested Applications IF/ICC, FC (Intra) Application Description
Tested Reactivity human, mouse, rat, pig
Immunogen RAD23B fusion protein Ag28658 种属同源性预测
Host / Isotype Mouse / IgG2a
Class Monoclonal
Type Antibody
Full Name RAD23 homolog B (S. cerevisiae)
Synonyms XP-C repair-complementing complex 58 kDa protein, p58, HR23B, HHR23B, 2E6G9
Calculated Molecular Weight 409 aa, 43 kDa
Observed Molecular Weight58 kDa
GenBank Accession NumberBC020973
Gene Symbol RAD23B
Gene ID (NCBI) 5887
Conjugate CoraLite® Plus 488 Fluorescent Dye
Excitation/Emission Maxima Wavelengths493 nm / 522 nm
Form Liquid
Purification MethodProtein A purification
UNIPROT IDP54727
Storage Buffer PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
Storage ConditionsStore at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

RAD23B, also named as p58, hHR23B and HR23B, belongs to the RAD23 family. It plays a central role both in proteosomal degradation of misfolded proteins and DNA repair. RAD23B is a central component of a complex required to couple deglycosylation and proteasome-mediated degradation of misfolded proteins in the endoplasmic reticulun that are retrotranslocated in the cytosol. Involved in DNA excision repair by stabilizing XPC protein. It may play a part in DNA damage recognition and/or in altering chromatin structure to allow access by damage-processing enzymes. RAD23B has two subunit: Heterodimer of a 125 kDa subunit (p125) and of a 58 kDa subunit (p58).

实验方案

Product Specific Protocols
IF protocol for CL Plus 488 RAD23B antibody CL488-67988Download protocol
FC protocol for CL Plus 488 RAD23B antibody CL488-67988Download protocol
Standard Protocols
Click here to view our Standard Protocols