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CoraLite® Plus 488-conjugated RAD50 Polyclonal antibody

RAD50 Polyclonal Antibody for IF/ICC, FC (Intra)

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

IF/ICC, FC (Intra)

Conjugate

CoraLite® Plus 488 Fluorescent Dye

Cat no : CL488-29390

Print datasheet

Synonyms

RAD50 homolog (S. cerevisiae), hRad50

验证数据展示

  • FC experiment of HeLa using CL488-29390

    FC experiment of HeLa using CL488-29390

    1X10^6 HeLa cells were intracellularly stained with 0.4 ug CoraLite® Plus 488 Anti-Human RAD50 (CL488-29390) (red), or 0.4 ug Control Antibody. Cells were fixed and permeabilized with Transcription Factor Staining Buffer Kit (PF00011).

  • IF Staining of U2OS using CL488-29390

    IF Staining of U2OS using CL488-29390

    Immunofluorescent analysis of (4% PFA) fixed U2OS cells using CoraLite® Plus 488 RAD50 antibody (CL488-29390) at dilution of 1:200.

经过测试的应用

Positive IF/ICC detected inU2OS cells
Positive FC (Intra) detected inHeLa cells
For other applications, we recommend the unconjugated version of this antibody, 29390-1-AP

推荐稀释比

ApplicationDilution
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

CL488-29390 targets RAD50 in IF/ICC, FC (Intra) applications and shows reactivity with human, mouse, rat samples.

Tested Applications IF/ICC, FC (Intra) Application Description
Tested Reactivity human, mouse, rat
Immunogen RAD50 fusion protein Ag29949 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name RAD50 homolog (S. cerevisiae)
Synonyms RAD50 homolog (S. cerevisiae), hRad50
Calculated Molecular Weight 154 kDa
Observed Molecular Weight154 kDa
GenBank Accession NumberNM_005732
Gene Symbol RAD50
Gene ID (NCBI) 10111
Conjugate CoraLite® Plus 488 Fluorescent Dye
Excitation/Emission Maxima Wavelengths493 nm / 522 nm
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ92878
Storage Buffer PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
Storage ConditionsStore at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

实验方案

Product Specific Protocols
IF protocol for CL Plus 488 RAD50 antibody CL488-29390Download protocol
FC protocol for CL Plus 488 RAD50 antibody CL488-29390Download protocol
Standard Protocols
Click here to view our Standard Protocols