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×
×
Host
0
Species Reactivity
0
Species Reactivity
0
Conjugate
0
Conjugate
0
Compatible Species
0
Conjugate
0

验证数据展示

  • WB analysis using 84537-5-RR (same clone as 84537-5-PBS)

    WB analysis using 84537-5-RR (same clone as 84537-5-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 84537-5-RR (RAD51C antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 84537-5-PBS in a different storage buffer formulation.

  • FC experiment of MCF-7 using 84537-5-RR (same clone as 84537-5-PBS)

    FC experiment of MCF-7 using 84537-5-RR (same clone as 84537-5-PBS)

    1x10^6 MCF-7 cells were intracellularly stained with 0.25 ug RAD51C Recombinant antibody (84537-5-RR, Clone:241971A11) and APC-Conjugated Goat Anti-Rabbit IgG(H+L)(red), or 0.25 ug Rabbit IgG Isotype Control Recombinant Antibody (98136-1-RR, Clone: 240953C9) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C). This data was developed using the same antibody clone with 84537-5-PBS in a different storage buffer formulation.

  • Affinity and Kinetic Characterization of 84537-5-RR

    Affinity and Kinetic Characterization of 84537-5-RR

    Biolayer interferometry (BLl) kinetic assays of 84537-5-RR against Human RAD51C were performed. The affinity constant is below 1 pM.

RAD51C Recombinant antibody, PBS Only

RAD51C Uni-rAbTM Recombinant Antibody for WB, FC (Intra), Indirect ELISA
Cat No. 84537-5-PBS

产品说明书

CloneNo. 241971A11

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse

应用

WB, FC (Intra), Indirect ELISA

别名

241971A11

缓冲液配方:  PBS Only
PBS and Azide
PBS Only
偶联物:  Unconjugated
规格: 

-/ -

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产品信息

84537-5-PBS targets RAD51C in WB, FC (Intra), Indirect ELISA applications and shows reactivity with human, mouse samples.

经测试应用 WB, FC (Intra), Indirect ELISA Application Description
经测试反应性 human, mouse
免疫原 RAD51C fusion protein Ag35070 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Recombinant
产品类型 Antibody
全称 RAD51 homolog C (S. cerevisiae)
别名 241971A11
计算分子量 376 aa, 42 kDa
观测分子量 40-42 kDa
GenBank蛋白编号BC107753
基因名称 RAD51C
Gene ID (NCBI) 5889
偶联类型 Unconjugated
形式 Liquid
纯化方式Protein A purfication
UNIPROT IDO43502
储存缓冲液 PBS only , pH 7.3
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

RAD51C (DNA repair protein RAD51 homolog 3) is essential for the homologous recombination (HR) pathway of DNA repair and is involved in the homologous recombination repair (HRR) pathway of double-stranded DNA breaks arising during DNA replication or induced by DNA-damaging agents (PMID: 14716019; 16215984; 16395335).