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RAE1 Recombinant antibody, PBS Only (Capture/Detector)

RAE1 Uni-rAbTM Recombinant Antibody for WB, FC (Intra), Cytometric bead array, Indirect ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, FC (Intra), Cytometric bead array, Indirect ELISA

Conjugate

Unconjugated

CloneNo.

240861E12

Cat no : 83712-1-PBS

Print datasheet

Synonyms

mRNA-associated protein mrnp 41, mRNA export factor RAE1, mRNA export factor, Mnrp41, MIG14

验证数据展示

  • WB analysis using 83712-1-RR (same clone as 83712-1-PBS)

    WB analysis using 83712-1-RR (same clone as 83712-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 83712-1-RR (RAE1 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 83712-1-PBS in a different storage buffer formulation.

  • FC experiment of HepG2 using 83712-1-RR (same clone as 83712-1-PBS)

    FC experiment of HepG2 using 83712-1-RR (same clone as 83712-1-PBS)

    1x10^6 HepG2 cells were intracellularly stained with 0.25 ug RAE1 Recombinant antibody (83712-1-RR, Clone:240861E12) and APC-Conjugated Goat Anti-Rabbit IgG(H+L)(red), or 0.25 ug Isotype Control (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C). This data was developed using the same antibody clone with 83712-1-PBS in a different storage buffer formulation.

  • Cytometric bead array standard curve of MP00683-1

    Cytometric bead array standard curve of MP00683-1

    Cytometric bead array standard curve of MP00683-1, RAE1 Recombinant Matched Antibody Pair, PBS Only. Capture antibody: 83712-3-PBS. Detection antibody: 83712-1-PBS. Standard: Ag12102. Range: 0.781-100 ng/mL.

  • Cytometric bead array standard curve of MP00683-3

    Cytometric bead array standard curve of MP00683-3

    Cytometric bead array standard curve of MP00683-3, RAE1 Recombinant Matched Antibody Pair, PBS Only. Capture antibody: 83712-1-PBS. Detection antibody: 83712-2-PBS. Standard: Ag12102. Range: 0.781-100 ng/mL.

  • Affinity and Kinetic Characterization of 83712-1-RR

    Affinity and Kinetic Characterization of 83712-1-RR

    Biolayer interferometry (BLl) kinetic assays of 83712-1-RR against Human RAE1 were performed. The affinity constant is 1.36 nM.

Planning an IHC experiment? We recommend our IHCeasy RAE1 Ready-To-Use IHC Kit. RAE1 primary antibody included.

产品信息

83712-1-PBS targets RAE1 as part of a matched antibody pair:

MP00683-1: 83712-3-PBS capture and 83712-1-PBS detection (validated in Cytometric bead array)

MP00683-3: 83712-1-PBS capture and 83712-2-PBS detection (validated in Cytometric bead array)

Unconjugated rabbit recombinant monoclonal antibody in PBS only (BSA and azide free) storage buffer at a concentration of 1 mg/mL, ready for conjugation. Created using Proteintech’s proprietary in-house recombinant technology. Recombinant production enables unrivalled batch-to-batch consistency, easy scale-up, and future security of supply.

This conjugation ready format makes antibodies ideal for use in many applications including: ELISAs, multiplex assays requiring matched pairs, mass cytometry, and multiplex imaging applications.Antibody use should be optimized by the end user for each application and assay.

Tested Applications WB, FC (Intra), Cytometric bead array, Indirect ELISA Application Description
Tested Reactivity human, mouse
Immunogen RAE1 fusion protein Ag12102 种属同源性预测
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Full Name RAE1 RNA export 1 homolog (S. pombe)
Synonyms mRNA-associated protein mrnp 41, mRNA export factor RAE1, mRNA export factor, Mnrp41, MIG14
Calculated Molecular Weight 368 aa, 41 kDa
Observed Molecular Weight41 kDa
GenBank Accession NumberBC103754
Gene Symbol RAE1
Gene ID (NCBI) 8480
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
UNIPROT IDP78406
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

RAE1 acts as a mRNA export factor involved in nucleocytoplasmic transport, it plays a role in mitotic bipolar spindle formation. (PMID: 33849972, MID: 17172455)