• All
  • Primary Antibodies
  • Conjugated Antibodies for IF
  • Conjugated Antibodies for FC
  • Secondary Antibodies
  • Antibody Labeling KitsNew
  • ELISA Kits
  • IHC Kits
  • Magnetic Cell Separation Kits
  • Cytokines & Growth Factors
  • Neutralizing/activating Antibodies
  • Nanobody-based Reagents
  • Accessory Products and Kits
  • Fusion Proteins
×
×
Host
0
Species Reactivity
0
Species Reactivity
0
Conjugate
0
Conjugate
0
Compatible Species
0
Conjugate
0

验证数据展示

  • WB analysis using 83712-1-RR (same clone as 83712-1-PBS)

    WB analysis using 83712-1-RR (same clone as 83712-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 83712-1-RR (RAE1 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 83712-1-PBS in a different storage buffer formulation.

  • FC experiment of HepG2 using 83712-1-RR (same clone as 83712-1-PBS)

    FC experiment of HepG2 using 83712-1-RR (same clone as 83712-1-PBS)

    1x10^6 HepG2 cells were intracellularly stained with 0.25 ug RAE1 Recombinant antibody (83712-1-RR, Clone:240861E12) and APC-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L)(red), or 0.25 ug Isotype Control (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C). This data was developed using the same antibody clone with 83712-1-PBS in a different storage buffer formulation.

  • Cytometric bead array standard curve of MP00683-1

    Cytometric bead array standard curve of MP00683-1

    Cytometric bead array standard curve of MP00683-1, RAE1 Recombinant Matched Antibody Pair, PBS Only. Capture antibody: 83712-3-PBS. Detection antibody: 83712-1-PBS. Standard: Ag12102. Range: 0.781-100 ng/mL.

  • Cytometric bead array standard curve of MP00683-3

    Cytometric bead array standard curve of MP00683-3

    Cytometric bead array standard curve of MP00683-3, RAE1 Recombinant Matched Antibody Pair, PBS Only. Capture antibody: 83712-1-PBS. Detection antibody: 83712-2-PBS. Standard: Ag12102. Range: 0.781-100 ng/mL.

  • Affinity and Kinetic Characterization of 83712-1-RR

    Affinity and Kinetic Characterization of 83712-1-RR

    Biolayer interferometry (BLl) kinetic assays of 83712-1-RR against Human RAE1 were performed. The affinity constant is 1.36 nM.

RAE1 Recombinant antibody, PBS Only (Capture/Detector)

RAE1 Uni-rAbTM Recombinant Antibody for WB, FC (Intra), Cytometric bead array, Indirect ELISA
Cat No. 83712-1-PBS

产品说明书

CloneNo. 240861E12

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse

应用

WB, FC (Intra), Cytometric bead array, Indirect ELISA

别名

mRNA-associated protein mrnp 41, mRNA export factor RAE1, mRNA export factor, Mnrp41, MIG14

缓冲液配方:  PBS Only
PBS and Azide
PBS Only
偶联物:  Unconjugated
规格: 

-/ -

点击咨询售前客服

点击咨询售后客服

Planning an IHC experiment? We recommend our IHCeasy RAE1 Ready-To-Use IHC Kit. RAE1 primary antibody included.

产品信息

83712-1-PBS targets RAE1 as part of a matched antibody pair:

MP00683-1: 83712-3-PBS capture and 83712-1-PBS detection (validated in Cytometric bead array)

MP00683-3: 83712-1-PBS capture and 83712-2-PBS detection (validated in Cytometric bead array)

Unconjugated rabbit recombinant monoclonal antibody in PBS only (BSA and azide free) storage buffer at a concentration of 1 mg/mL, ready for conjugation. Created using Proteintech’s proprietary in-house recombinant technology. Recombinant production enables unrivalled batch-to-batch consistency, easy scale-up, and future security of supply.

This conjugation ready format makes antibodies ideal for use in many applications including: ELISAs, multiplex assays requiring matched pairs, mass cytometry, and multiplex imaging applications.Antibody use should be optimized by the end user for each application and assay.

经测试应用 WB, FC (Intra), Cytometric bead array, Indirect ELISA Application Description
经测试反应性 human, mouse
免疫原 RAE1 fusion protein Ag12102 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Recombinant
产品类型 Antibody
全称 RAE1 RNA export 1 homolog (S. pombe)
别名 mRNA-associated protein mrnp 41, mRNA export factor RAE1, mRNA export factor, Mnrp41, MIG14
计算分子量 368 aa, 41 kDa
观测分子量41 kDa
GenBank蛋白编号BC103754
基因名称 RAE1
Gene ID (NCBI) 8480
偶联类型 Unconjugated
形式 Liquid
纯化方式Protein A purification
UNIPROT IDP78406
储存缓冲液 PBS Only
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

RAE1 acts as a mRNA export factor involved in nucleocytoplasmic transport, it plays a role in mitotic bipolar spindle formation. (PMID: 33849972, MID: 17172455)