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验证数据展示

  • WB analysis using 83712-1-RR

    WB analysis using 83712-1-RR

    Various lysates were subjected to SDS PAGE followed by western blot with 83712-1-RR (RAE1 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

  • FC experiment of HepG2 using 83712-1-RR

    FC experiment of HepG2 using 83712-1-RR

    1x10^6 HepG2 cells were intracellularly stained with 0.25 ug RAE1 Recombinant antibody (83712-1-RR, Clone:240861E12) and APC-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L)(red), or 0.25 ug Isotype Control (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

  • Affinity and Kinetic Characterization of 83712-1-RR

    Affinity and Kinetic Characterization of 83712-1-RR

    Biolayer interferometry (BLl) kinetic assays of 83712-1-RR against Human RAE1 were performed. The affinity constant is 1.36 nM.

RAE1 Recombinant antibody

RAE1 Uni-rAbTM Recombinant Antibody for WB, FC (Intra), ELISA
Cat No. 83712-1-RR

CloneNo.

240861E12

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, FC (Intra), ELISA

Synonyms

mRNA-associated protein mrnp 41, mRNA export factor RAE1, mRNA export factor, Mnrp41, MIG14

Formulation:  PBS and Azide
PBS and Azide
PBS Only
Conjugate:  Unconjugated
Unconjugated
Size: 

-/ -

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经过测试的应用

Positive WB detected inHepG2 cells, MCF-7 cells, HeLa cells, HEK-293 cells, BxPC-3 cells, mouse brain tissue, mouse thymus tissue
Positive FC (Intra) detected inHepG2 cells
Planning an IHC experiment? We recommend our IHCeasy RAE1 Ready-To-Use IHC Kit. RAE1 primary antibody included.

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:5000-1:50000
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.25 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

83712-1-RR targets RAE1 in WB, FC (Intra), ELISA applications and shows reactivity with human, mouse samples.

Tested Applications WB, FC (Intra), ELISA Application Description
Tested Reactivity human, mouse
Immunogen RAE1 fusion protein Ag12102 种属同源性预测
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Full Name RAE1 RNA export 1 homolog (S. pombe)
Synonyms mRNA-associated protein mrnp 41, mRNA export factor RAE1, mRNA export factor, Mnrp41, MIG14
Calculated Molecular Weight 368 aa, 41 kDa
Observed Molecular Weight41 kDa
GenBank Accession NumberBC103754
Gene Symbol RAE1
Gene ID (NCBI) 8480
RRIDAB_3671315
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
UNIPROT IDP78406
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

RAE1 acts as a mRNA export factor involved in nucleocytoplasmic transport, it plays a role in mitotic bipolar spindle formation. (PMID: 33849972, MID: 17172455)

实验方案

Product Specific Protocols
WB protocol for RAE1 antibody 83712-1-RRDownload protocol
FC protocol for RAE1 antibody 83712-1-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols