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验证数据展示

  • WB analysis using 83712-4-RR (same clone as 83712-4-PBS)

    WB analysis using 83712-4-RR (same clone as 83712-4-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 83712-4-RR (RAE1 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 83712-4-PBS in a different storage buffer formulation.

  • IHC staining of mouse testis using 83712-4-RR (same clone as 83712-4-PBS)

    IHC staining of mouse testis using 83712-4-RR (same clone as 83712-4-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 83712-4-RR (RAE1 antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 83712-4-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with RAE1 antibody included
  • IF Staining of HepG2 using 83712-4-RR (same clone as 83712-4-PBS)

    IF Staining of HepG2 using 83712-4-RR (same clone as 83712-4-PBS)

    Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using RAE1 antibody (83712-4-RR, Clone: 240861B7 ) at dilution of 1:250 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red). This data was developed using the same antibody clone with 83712-4-PBS in a different storage buffer formulation.

  • Affinity and Kinetic Characterization of 83712-4-RR

    Affinity and Kinetic Characterization of 83712-4-RR

    Biolayer interferometry (BLl) kinetic assays of 83712-4-RR against Human RAE1 were performed. The affinity constant is 20.7 nM.

RAE1 Recombinant antibody, PBS Only

RAE1 Uni-rAbTM Recombinant Antibody for WB, IHC, IF/ICC, ELISA
Cat No. 83712-4-PBS

产品说明书

CloneNo. 240861B7

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse

应用

WB, IHC, IF/ICC, ELISA

别名

mRNA-associated protein mrnp 41, mRNA export factor RAE1, mRNA export factor, Mnrp41, MIG14

缓冲液配方:  PBS Only
PBS and Azide
PBS Only
偶联物:  Unconjugated
规格: 

-/ -

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Planning an IHC experiment? We recommend our IHCeasy RAE1 Ready-To-Use IHC Kit. RAE1 primary antibody included.

产品信息

83712-4-PBS targets RAE1 in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human, mouse samples.

经测试应用 WB, IHC, IF/ICC, ELISA Application Description
经测试反应性 human, mouse
免疫原 RAE1 fusion protein Ag12102 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Recombinant
产品类型 Antibody
全称 RAE1 RNA export 1 homolog (S. pombe)
别名 mRNA-associated protein mrnp 41, mRNA export factor RAE1, mRNA export factor, Mnrp41, MIG14
计算分子量 368 aa, 41 kDa
GenBank蛋白编号BC103754
基因名称 RAE1
Gene ID (NCBI) 8480
偶联类型 Unconjugated
形式 Liquid
纯化方式Protein A purfication
UNIPROT IDP78406
储存缓冲液 PBS Only
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

RAE1 acts as a mRNA export factor involved in nucleocytoplasmic transport, it plays a role in mitotic bipolar spindle formation. (PMID: 33849972, MID: 17172455)