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验证数据展示

  • WB analysis using 66592-1-Ig (same clone as 66592-1-PBS)

    WB analysis using 66592-1-Ig (same clone as 66592-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 66592-1-Ig (RAF1 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours. The membrane was stripped and reblotted with HRP-conjugated GAPDH Monoclonal antibody (HRP-60004) as loading control. This data was developed using the same antibody clone with 66592-1-PBS in a different storage buffer formulation.

  • IHC staining of human prostate cancer using 66592-1-Ig (same clone as 66592-1-PBS)

    IHC staining of human prostate cancer using 66592-1-Ig (same clone as 66592-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human prostate cancer tissue slide using 66592-1-Ig (RAF1 antibody) at dilution of 1:300 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66592-1-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with RAF1 antibody included
  • IHC staining of human prostate cancer using 66592-1-Ig (same clone as 66592-1-PBS)

    IHC staining of human prostate cancer using 66592-1-Ig (same clone as 66592-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human prostate cancer tissue slide using 66592-1-Ig (RAF1 antibody) at dilution of 1:300 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66592-1-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with RAF1 antibody included
  • WB analysis of HEK-293 using 66592-1-Ig (same clone as 66592-1-PBS)

    WB analysis of HEK-293 using 66592-1-Ig (same clone as 66592-1-PBS)

    WB result of RAF1 antibody (66592-1-Ig; 1:2000; incubated at room temperature for 1.5 hours) with sh-Control and sh-RAF1 transfected HEK-293 cells. This data was developed using the same antibody clone with 66592-1-PBS in a different storage buffer formulation.

  • IF Staining of HEK-293 using 66592-1-Ig (same clone as 66592-1-PBS)

    IF Staining of HEK-293 using 66592-1-Ig (same clone as 66592-1-PBS)

    Immunofluorescent analysis of (-20°C Ethanol) fixed HEK-293 cells using RAF1 antibody (66592-1-Ig, Clone: 1D6A1 ) at dilution of 1:800 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L) (SA00013-1). This data was developed using the same antibody clone with 66592-1-PBS in a different storage buffer formulation.

  • Featured Product
  • KD/KO验证

RAF1 Monoclonal antibody, PBS Only

RAF1 Monoclonal Antibody for WB, IHC, IF/ICC, ELISA
Cat No. 66592-1-PBS

产品说明书

CloneNo. 1D6A1

宿主/亚型

Mouse / IgG2a

种属反应性

human, mouse

应用

WB, IHC, IF/ICC, ELISA

别名

Proto oncogene c RAF, NS5, EC:2.7.11.1, CRAF, c Raf

缓冲液配方:  PBS Only
PBS and Azide
PBS Only
偶联物:  Unconjugated
规格: 

-/ -

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Planning an IHC experiment? We recommend our IHCeasy RAF1 Ready-To-Use IHC Kit. RAF1 primary antibody included.

产品信息

66592-1-PBS targets RAF1 in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human, mouse samples.

经测试应用 WB, IHC, IF/ICC, ELISA Application Description
经测试反应性 human, mouse
免疫原 RAF1 fusion protein Ag25423 种属同源性预测
宿主/亚型 Mouse / IgG2a
抗体类别 Monoclonal
产品类型 Antibody
全称 v-raf-1 murine leukemia viral oncogene homolog 1
别名 Proto oncogene c RAF, NS5, EC:2.7.11.1, CRAF, c Raf
计算分子量 648 aa, 73 kDa
观测分子量 70-75 kDa
GenBank蛋白编号BC018119
基因名称 RAF1
Gene ID (NCBI) 5894
RRIDAB_2881952
偶联类型 Unconjugated
形式 Liquid
纯化方式Protein A purification
UNIPROT IDP04049
储存缓冲液 PBS only , pH 7.3
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

Raf-1 proto-oncogene, serine/threonine kinase(RAF1), is a MAP kinase kinase kinase (MAP3K), which functions downstream of the Ras family of membrane associated GTPases to which it binds directly. RAF1 has two isoforms with MW of 73, 75 kDa. RAF1 plays an important role in the control of gene expression involved in the cell division cycle, apoptosis, cell differentiation and cell migration.