- Featured Product
- KD/KO Validated
RAF1 Monoclonal antibody
RAF1 Monoclonal Antibody for IF/ICC, IHC, WB, ELISA
Host / Isotype
Mouse / IgG2a
Reactivity
Human, Mouse and More (2)
Applications
IF/ICC, IHC, WB, ELISA
Conjugate
Unconjugated
CloneNo.
1D6A1
验证数据展示
经过测试的应用
Positive WB detected in | HepG2 cells, DC2.4 cells, HEK-293 cells |
Positive IHC detected in | human prostate cancer tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
Positive IF detected in | HEK-293 cells |
推荐稀释比
Application | Dilution |
---|---|
Western Blot (WB) | WB : 1:1000-1:6000 |
Immunohistochemistry (IHC) | IHC : 1:150-1:600 |
Immunofluorescence (IF) | IF : 1:400-1:1600 |
It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
Sample-dependent, Check data in validation data gallery. |
发表文章中的应用
WB | See 7 publications below |
IF | See 1 publications below |
产品信息
66592-1-Ig targets RAF1 in WB, IF, IHC, ELISA applications and shows reactivity with Human, Mouse samples.
Tested Applications | IF/ICC, IHC, WB, ELISA Application Description |
Cited Applications | WB, IF |
Tested Reactivity | Human, Mouse |
Cited Reactivity | human, mouse |
Immunogen | RAF1 fusion protein Ag25423 种属同源性预测 |
Host / Isotype | Mouse / IgG2a |
Class | Monoclonal |
Type | Antibody |
Full Name | v-raf-1 murine leukemia viral oncogene homolog 1 |
Synonyms | c Raf, CRAF, NS5, Proto oncogene c RAF, RAF, Raf 1, RAF1 |
Calculated Molecular Weight | 648 aa, 73 kDa |
Observed Molecular Weight | 70-75 kDa |
GenBank Accession Number | BC018119 |
Gene Symbol | RAF1 |
Gene ID (NCBI) | 5894 |
RRID | AB_2881952 |
Conjugate | Unconjugated |
Form | Liquid |
Purification Method | Protein A purification |
UNIPROT ID | P04049 |
Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
实验方案
Product Specific Protocols | |
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WB protocol for RAF1 antibody 66592-1-Ig | Download protocol |
IHC protocol for RAF1 antibody 66592-1-Ig | Download protocol |
IF protocol for RAF1 antibody 66592-1-Ig | Download protocol |
Standard Protocols | |
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Click here to view our Standard Protocols |
发表文章
Species | Application | Title |
---|---|---|
World J Gastroenterol TATA-box-binding protein-associated factor 15 is a novel biomarker that promotes cell proliferation and migration in gastrointestinal stromal tumor | ||
Cancer Cell Int 4,5-Dimethoxycanthin-6-one is a novel LSD1 inhibitor that inhibits proliferation of glioblastoma cells and induces apoptosis and pyroptosis. | ||
World J Gastrointest Oncol Lnc524369 promotes hepatocellular carcinoma progression and predicts poor survival by activating YWHAZ-RAF1 signaling. | ||
Curr Med Sci Huai Qi Huang-induced Apoptosis via Down-regulating PRKCH and Inhibiting RAF/MEK/ERK Pathway in Ph+ Leukemia Cells. | ||
Cell Signal Integration analysis of cell division cycle-associated family genes revealed potential mechanisms of gliomagenesis and constructed an artificial intelligence-driven prognostic signature | ||
Proc Natl Acad Sci U S A Tumor necrosis factor-like cytokine 1A plays a role in inflammatory bowel disease pathogenesis |