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CoraLite®594-conjugated RAF1 Monoclonal antibody

RAF1 Monoclonal Antibody for IF/ICC
Cat No. CL594-66592

产品说明书

CloneNo. 1D6A1

宿主/亚型

Mouse / IgG2a

种属反应性

Human, Mouse

应用

IF/ICC

c Raf, CRAF, NS5, Proto oncogene c RAF, RAF, Raf 1, RAF1

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  CoraLite®594
规格: 

-/ -


经过测试的应用

Positive IF/ICC detected inHEK-293 cells
Planning an IHC experiment? We recommend our IHCeasy RAF1 Ready-To-Use IHC Kit. RAF1 primary antibody included.
For other applications, we recommend the unconjugated version of this antibody, 66592-1-Ig

推荐稀释比

应用推荐稀释比
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

CL594-66592 targets RAF1 in IF/ICC applications and shows reactivity with Human, Mouse samples.

经测试应用 IF/ICC Application Description
经测试反应性 Human, Mouse
免疫原 RAF1 fusion protein Ag25423 种属同源性预测
宿主/亚型 Mouse / IgG2a
抗体类别 Monoclonal
产品类型 Antibody
全称 v-raf-1 murine leukemia viral oncogene homolog 1
别名 c Raf, CRAF, NS5, Proto oncogene c RAF, RAF, Raf 1, RAF1
计算分子量 648 aa, 73 kDa
观测分子量 70-75 kDa
GenBank蛋白编号BC018119
基因名称 RAF1
Gene ID (NCBI) 5894
RRIDAB_2920013
偶联类型 CoraLite®594 Fluorescent Dye
最大激发/发射波长588 nm / 604 nm
形式 Liquid
纯化方式Protein A purification
UNIPROT IDP04049
储存缓冲液 PBS with 50% glycerol, 0.05% Proclin300, 0.5% BSA , pH 7.3
储存条件Store at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

Raf-1 proto-oncogene, serine/threonine kinase(RAF1), is a MAP kinase kinase kinase (MAP3K), which functions downstream of the Ras family of membrane associated GTPases to which it binds directly. RAF1 has two isoforms with MW of 73, 75 kDa. RAF1 plays an important role in the control of gene expression involved in the cell division cycle, apoptosis, cell differentiation and cell migration.

实验方案

Product Specific Protocols
IF protocol for CL594 RAF1 antibody CL594-66592Download protocol
Standard Protocols
Click here to view our Standard Protocols
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