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验证数据展示

  • IF Staining of HEK-293 using CL594-66592

    IF Staining of HEK-293 using CL594-66592

    Immunofluorescent analysis of (-20°C Ethanol) fixed HEK-293 cells using CoraLite®®594 RAF1 antibody (CL594-66592, Clone: 1D6A1 ) at dilution of 1:200.

  • Featured Product
  • KD/KO Validated

CoraLite®594-conjugated RAF1 Monoclonal antibody

RAF1 Monoclonal Antibody for IF/ICC
Cat No. CL594-66592

Print datasheet

CloneNo. 1D6A1

Host / Isotype

Mouse / IgG2a

Reactivity

Human, Mouse

Applications

IF/ICC

Synonyms

c Raf, CRAF, NS5, Proto oncogene c RAF, RAF, Raf 1, RAF1

Formulation:  PBS and Azide
PBS and Azide
Conjugate:  CoraLite®594
Size: 

-/ -

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经过测试的应用

Positive IF/ICC detected inHEK-293 cells
Planning an IHC experiment? We recommend our IHCeasy RAF1 Ready-To-Use IHC Kit. RAF1 primary antibody included.
For other applications, we recommend the unconjugated version of this antibody, 66592-1-Ig

推荐稀释比

ApplicationDilution
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

CL594-66592 targets RAF1 in IF/ICC applications and shows reactivity with Human, Mouse samples.

Tested Applications IF/ICC Application Description
Tested Reactivity Human, Mouse
Immunogen RAF1 fusion protein Ag25423 种属同源性预测
Host / Isotype Mouse / IgG2a
Class Monoclonal
Type Antibody
Full Name v-raf-1 murine leukemia viral oncogene homolog 1
Synonyms c Raf, CRAF, NS5, Proto oncogene c RAF, RAF, Raf 1, RAF1
Calculated Molecular Weight 648 aa, 73 kDa
Observed Molecular Weight 70-75 kDa
GenBank Accession NumberBC018119
Gene Symbol RAF1
Gene ID (NCBI) 5894
RRIDAB_2920013
Conjugate CoraLite®594 Fluorescent Dye
Excitation/Emission Maxima Wavelengths588 nm / 604 nm
Form Liquid
Purification MethodProtein A purification
UNIPROT IDP04049
Storage Buffer PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
Storage ConditionsStore at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

Raf-1 proto-oncogene, serine/threonine kinase(RAF1), is a MAP kinase kinase kinase (MAP3K), which functions downstream of the Ras family of membrane associated GTPases to which it binds directly. RAF1 has two isoforms with MW of 73, 75 kDa. RAF1 plays an important role in the control of gene expression involved in the cell division cycle, apoptosis, cell differentiation and cell migration.

实验方案

Product Specific Protocols
IF protocol for CL594 RAF1 antibody CL594-66592Download protocol
Standard Protocols
Click here to view our Standard Protocols