RAG1 Monoclonal antibody
RAG1 Monoclonal Antibody for WB, ELISA
Host / Isotype
Mouse / IgG2b
Reactivity
Human, mouse
Applications
WB, ELISA
Conjugate
Unconjugated
CloneNo.
3A1A4
验证数据展示
经过测试的应用
| Positive WB detected in | mouse thymus tissue |
推荐稀释比
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:2000-1:10000 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
68591-1-Ig targets RAG1 in WB, ELISA applications and shows reactivity with Human, mouse samples.
| Tested Applications | WB, ELISA Application Description |
| Tested Reactivity | Human, mouse |
| Immunogen | RAG1 fusion protein Ag30192 种属同源性预测 |
| Host / Isotype | Mouse / IgG2b |
| Class | Monoclonal |
| Type | Antibody |
| Full Name | recombination activating gene 1 |
| Synonyms | RAG 1, RAG1, RNF74 |
| Calculated Molecular Weight | 119 kDa |
| Observed Molecular Weight | 110-120 kDa |
| GenBank Accession Number | NM_000448 |
| Gene Symbol | RAG1 |
| Gene ID (NCBI) | 5896 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Protein A purification |
| UNIPROT ID | P15918 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
Recombination-activating gene (RAG) proteins include RAG1 and RAG2, which are crucial for Ig and T lymphocyte receptor (TCR) fragment rearrangement and are responsible for DNA recognition and cleavage at specific sequences. Mutations in RAG1 result in the complete or partial loss of recombinant enzyme activity, in unbalanced variable-diversity-joining (V(D)J) recombination, and in the impairment of T and B lymphocyte development at the early stages.
实验方案
| Product Specific Protocols | |
|---|---|
| WB protocol for RAG1 antibody 68591-1-Ig | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |