验证数据展示
产品信息
66998-1-PBS targets RAG2 in WB, IF/ICC, FC (Intra), Indirect ELISA applications and shows reactivity with Human, pig samples.
| 经测试应用 | WB, IF/ICC, FC (Intra), Indirect ELISA Application Description |
| 经测试反应性 | Human, pig |
| 免疫原 | RAG2 fusion protein Ag16780 种属同源性预测 |
| 宿主/亚型 | Mouse / IgG1 |
| 抗体类别 | Monoclonal |
| 产品类型 | Antibody |
| 全称 | recombination activating gene 2 |
| 别名 | V(D)J recombination-activating protein 2, RAG-2, RAG 2, 1B4E5 |
| 计算分子量 | 527 aa, 59 kDa |
| 观测分子量 | 57-62 kDa |
| GenBank蛋白编号 | BC022397 |
| 基因名称 | RAG2 |
| Gene ID (NCBI) | 5897 |
| RRID | AB_2882315 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Protein A purification |
| UNIPROT ID | P55895 |
| 储存缓冲液 | PBS only , pH 7.3 |
| 储存条件 | Store at -80°C. The product is shipped with ice packs. Upon receipt, store it immediately at -80°C |
背景介绍
Recombination activating gene 2(RAG2) is core part of the RAG complex(RAG1 and RAG2), which mediates the DNA cleavage phase during V(D)J recombination. The RAG complex also plays a role in pre-B cell allelic exclusion, a process leading to expression of a single immunoglobulin heavy chain allele to enforce clonality and monospecific recognition by the B-cell antigen receptor (BCR) expressed on individual B-lymphocytes. The introduction of DNA breaks by the RAG complex on one immunoglobulin allele induces ATM-dependent repositioning of the other allele to pericentromeric heterochromatin, preventing accessibility to the RAG complex and recombination of the second allele. In the RAG complex, RAG2 is not the catalytic component but is required for all known catalytic activities mediated by RAG1. It probably acts as a sensor of chromatin state that recruits the RAG complex to H3K4me3