CoraLite® Plus 488-conjugated RAG2 Monoclonal antibody

RAG2 Monoclonal Antibody for IF/ICC, FC (Intra)
Cat No. CL488-66998

产品说明书

CloneNo. 1B4E5

宿主/亚型

Mouse / IgG1

种属反应性

Human, pig

应用

IF/ICC, FC (Intra)

V(D)J recombination-activating protein 2, RAG-2, RAG 2, 1B4E5

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  CoraLite® Plus 488
规格: 

-/ -


经过测试的应用

Positive IF/ICC detected inHepG2 cells
Positive FC (Intra) detected inHepG2 cells
For other applications, we recommend the unconjugated version of this antibody, 66998-1-Ig

推荐稀释比

应用推荐稀释比
Immunofluorescence (IF)/ICCIF/ICC : 1:300-1:1200
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

CL488-66998 targets RAG2 in IF/ICC, FC (Intra) applications and shows reactivity with Human, pig samples.

经测试应用 IF/ICC, FC (Intra) Application Description
经测试反应性 Human, pig
免疫原 RAG2 fusion protein Ag16780 种属同源性预测
宿主/亚型 Mouse / IgG1
抗体类别 Monoclonal
产品类型 Antibody
全称 recombination activating gene 2
别名 V(D)J recombination-activating protein 2, RAG-2, RAG 2, 1B4E5
计算分子量 527 aa, 59 kDa
GenBank蛋白编号BC022397
基因名称 RAG2
Gene ID (NCBI) 5897
RRIDAB_3672933
偶联类型 CoraLite® Plus 488 Fluorescent Dye
最大激发/发射波长493 nm / 522 nm
形式 Liquid
纯化方式Protein G purification
UNIPROT IDP55895
储存缓冲液 PBS with 50% glycerol, 0.05% Proclin300, 0.5% BSA , pH 7.3
储存条件Store at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

Recombination activating gene 2(RAG2) is core part of the RAG complex(RAG1 and RAG2), which mediates the DNA cleavage phase during V(D)J recombination. The RAG complex also plays a role in pre-B cell allelic exclusion, a process leading to expression of a single immunoglobulin heavy chain allele to enforce clonality and monospecific recognition by the B-cell antigen receptor (BCR) expressed on individual B-lymphocytes. The introduction of DNA breaks by the RAG complex on one immunoglobulin allele induces ATM-dependent repositioning of the other allele to pericentromeric heterochromatin, preventing accessibility to the RAG complex and recombination of the second allele. In the RAG complex, RAG2 is not the catalytic component but is required for all known catalytic activities mediated by RAG1. It probably acts as a sensor of chromatin state that recruits the RAG complex to H3K4me3

实验方案

Product Specific Protocols
IF protocol for CL Plus 488 RAG2 antibody CL488-66998Download protocol
FC protocol for CL Plus 488 RAG2 antibody CL488-66998Download protocol
Standard Protocols
Click here to view our Standard Protocols
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