验证数据展示
经过测试的应用
| Positive IF/ICC detected in | HepG2 cells |
| Positive FC (Intra) detected in | HepG2 cells |
推荐稀释比
| Application | Dilution |
|---|---|
| Immunofluorescence (IF)/ICC | IF/ICC : 1:300-1:1200 |
| Flow Cytometry (FC) (INTRA) | FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
CL488-66998 targets RAG2 in IF/ICC, FC (Intra) applications and shows reactivity with Human, pig samples.
| Tested Applications | IF/ICC, FC (Intra) Application Description |
| Tested Reactivity | Human, pig |
| Immunogen | RAG2 fusion protein Ag16780 种属同源性预测 |
| Host / Isotype | Mouse / IgG1 |
| Class | Monoclonal |
| Type | Antibody |
| Full Name | recombination activating gene 2 |
| Synonyms | V(D)J recombination-activating protein 2, RAG-2, RAG 2, 1B4E5 |
| Calculated Molecular Weight | 527 aa, 59 kDa |
| GenBank Accession Number | BC022397 |
| Gene Symbol | RAG2 |
| Gene ID (NCBI) | 5897 |
| RRID | AB_3672933 |
| Conjugate | CoraLite® Plus 488 Fluorescent Dye |
| Excitation/Emission Maxima Wavelengths | 493 nm / 522 nm |
| Form | Liquid |
| Purification Method | Protein G purification |
| UNIPROT ID | P55895 |
| Storage Buffer | PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3. |
| Storage Conditions | Store at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
Recombination activating gene 2(RAG2) is core part of the RAG complex(RAG1 and RAG2), which mediates the DNA cleavage phase during V(D)J recombination. The RAG complex also plays a role in pre-B cell allelic exclusion, a process leading to expression of a single immunoglobulin heavy chain allele to enforce clonality and monospecific recognition by the B-cell antigen receptor (BCR) expressed on individual B-lymphocytes. The introduction of DNA breaks by the RAG complex on one immunoglobulin allele induces ATM-dependent repositioning of the other allele to pericentromeric heterochromatin, preventing accessibility to the RAG complex and recombination of the second allele. In the RAG complex, RAG2 is not the catalytic component but is required for all known catalytic activities mediated by RAG1. It probably acts as a sensor of chromatin state that recruits the RAG complex to H3K4me3
实验方案
| Product Specific Protocols | |
|---|---|
| IF protocol for CL Plus 488 RAG2 antibody CL488-66998 | Download protocol |
| FC protocol for CL Plus 488 RAG2 antibody CL488-66998 | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |