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Ager/Rage Recombinant antibody

Ager/Rage Uni-rAbTM Recombinant Antibody for WB, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

CloneNo.

240774F11

Cat no : 83742-5-RR

Print datasheet

Synonyms

Ager, RAGE, RAGE1, RAGE 1, MOK

验证数据展示

  • WB analysis using 83742-5-RR

    WB analysis using 83742-5-RR

    Various lysates were subjected to SDS PAGE followed by western blot with 83742-5-RR (RAGE antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

  • IHC staining of mouse lung using 83742-5-RR

    IHC staining of mouse lung using 83742-5-RR

    Immunohistochemical analysis of paraffin-embedded mouse lung tissue slide using 83742-5-RR (Ager/Rage antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Affinity and Kinetic Characterization of 83742-5-RR

    Affinity and Kinetic Characterization of 83742-5-RR

    Biolayer interferometry (BLl) kinetic assays of 83608-4-RR against Human ATM were performed. The affinity constant is 2.58 nM. Biolayer interferometry (BLl) kinetic assays of 83608-4-RR against Mouse RAGE were performed. The affinity constant is 6.41 nM.

经过测试的应用

Positive WB detected inrat lung tissue, mouse lung tissue
Positive IHC detected inmouse lung tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:5000-1:50000
Immunohistochemistry (IHC)IHC : 1:250-1:1000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

The immunogen of 83742-5-RR is Ager/Rage Fusion Protein expressed in E. coli.

Tested Applications WB, IHC, ELISA Application Description
Tested Reactivity human, mouse, rat
Immunogen Fusion Protein 种属同源性预测
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Full Name advanced glycosylation end product-specific receptor
Synonyms Ager, RAGE, RAGE1, RAGE 1, MOK
Calculated Molecular Weight43 kDa
Observed Molecular Weight42-55 kDa
GenBank Accession NumberNM_007425.3
Gene Symbol Ager
Gene ID (NCBI) 11596
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purfication
UNIPROT IDQ62151-1
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

The receptor for advanced glycation end products (RAGE) is a cell surface transmembrane multiligand receptor, also known as Ager, is a multiligand member of the immunoglobulin superfamily. It has many isoforms and is predominantly expressed in the lung. AGER expression is upregulated in many diseases such as Alzheimer's disease, diabetes mellitus, atherosclerosis, and rheumatoid arthritis (PMID: 35099614).

实验方案

Product Specific Protocols
WB protocol for Ager/Rage antibody 83742-5-RRDownload protocol
IHC protocol for Ager/Rage antibody 83742-5-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols