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验证数据展示

  • FC experiment of MCF-7 using CL594-67093

    FC experiment of MCF-7 using CL594-67093

    1X10^6 MCF-7 cells were intracellularly stained with 0.8 ug CoraLite®594 Anti-Human RALA (CL594-67093, Clone:1H9H5) (red), or 0.8 ug CoraLite®594 Mouse IgG2a Isotype Control (C1.18.4) (CL594-65208, Clone: C1.18.4) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

  • Featured Product
  • KD/KO验证

CoraLite®594-conjugated RALA Monoclonal antibody

RALA Monoclonal Antibody for FC (Intra)
Cat No. CL594-67093

产品说明书

CloneNo. 1H9H5

宿主/亚型

Mouse / IgG2a

种属反应性

Human

应用

FC (Intra)

别名

Ras-related protein Ral-A, Ras related protein Ral A, RAL, EC:3.6.5.2, 1H9H5

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  CoraLite®594
规格: 

-/ -

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经过测试的应用

Positive FC (Intra) detected inMCF-7 cells
Planning an IHC experiment? We recommend our IHCeasy RALA Ready-To-Use IHC Kit. RALA primary antibody included.
For other applications, we recommend the unconjugated version of this antibody, 67093-1-Ig

推荐稀释比

应用推荐稀释比
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.80 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

CL594-67093 targets RALA in FC (Intra) applications and shows reactivity with Human samples.

经测试应用 FC (Intra) Application Description
经测试反应性 Human
免疫原 RALA fusion protein Ag5329 种属同源性预测
宿主/亚型 Mouse / IgG2a
抗体类别 Monoclonal
产品类型 Antibody
全称 v-ral simian leukemia viral oncogene homolog A (ras related)
别名 Ras-related protein Ral-A, Ras related protein Ral A, RAL, EC:3.6.5.2, 1H9H5
计算分子量 206 aa, 24 kDa
观测分子量 24 kDa
GenBank蛋白编号BC039858
基因名称 RALA
Gene ID (NCBI) 5898
RRIDAB_3084746
偶联类型 CoraLite®594 Fluorescent Dye
最大激发/发射波长588 nm / 604 nm
形式 Liquid
纯化方式Protein A purification
UNIPROT IDP11233
储存缓冲液 PBS with 50% glycerol, 0.05% Proclin300, 0.5% BSA , pH 7.3
储存条件Store at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

实验方案

Product Specific Protocols
FC protocol for CL594 RALA antibody CL594-67093Download protocol
Standard Protocols
Click here to view our Standard Protocols