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CoraLite® Plus 488-conjugated RALBP1 Monoclonal antibody

RALBP1 Monoclonal Antibody for IF/ICC, FC (Intra)

Host / Isotype

Mouse / IgG1

Reactivity

human, mouse

Applications

IF/ICC, FC (Intra)

Conjugate

CoraLite® Plus 488 Fluorescent Dye

CloneNo.

1B2F5

Cat no : CL488-67359

Print datasheet

Synonyms

DNP-SG ATPase, DNP SG ATPase, Dinitrophenyl S-glutathione ATPase, 76 kDa Ral-interacting protein, 76 kDa Ral interacting protein

验证数据展示

  • FC experiment of HEK-293 using CL488-67359

    FC experiment of HEK-293 using CL488-67359

    1X10^6 HEK-293 cells were intracellularly stained with 0.4 ug CoraLite® Plus 488 Anti-Human RALBP1 (CL488-67359, Clone:1B2F5) (red), or 0.4 ug Control Antibody. Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

  • IF Staining of A431 using CL488-67359

    IF Staining of A431 using CL488-67359

    Immunofluorescent analysis of (-20°C Methanol) fixed A431 cells using CoraLite® Plus 488 RALBP1 antibody (CL488-67359, Clone: 1B2F5 ) at dilution of 1:200.

经过测试的应用

Positive IF/ICC detected inA431 cells
Positive FC (Intra) detected inHEK-293 cells
Planning an IHC experiment? We recommend our IHCeasy RALBP1 Ready-To-Use IHC Kit. RALBP1 primary antibody included.
For other applications, we recommend the unconjugated version of this antibody, 67359-1-Ig

推荐稀释比

ApplicationDilution
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

CL488-67359 targets RALBP1 in IF/ICC, FC (Intra) applications and shows reactivity with human, mouse samples.

Tested Applications IF/ICC, FC (Intra) Application Description
Tested Reactivity human, mouse
Immunogen RALBP1 fusion protein Ag28618 种属同源性预测
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Full Name ralA binding protein 1
Synonyms DNP-SG ATPase, DNP SG ATPase, Dinitrophenyl S-glutathione ATPase, 76 kDa Ral-interacting protein, 76 kDa Ral interacting protein
Calculated Molecular Weight 655 aa, 76 kDa
Observed Molecular Weight 95 kDa
GenBank Accession NumberBC013126
Gene Symbol RALBP1
Gene ID (NCBI) 10928
Conjugate CoraLite® Plus 488 Fluorescent Dye
Excitation/Emission Maxima Wavelengths493 nm / 522 nm
Form Liquid
Purification MethodProtein G purification
UNIPROT IDQ15311
Storage Buffer PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
Storage ConditionsStore at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

实验方案

Product Specific Protocols
IF protocol for CL Plus 488 RALBP1 antibody CL488-67359Download protocol
Standard Protocols
Click here to view our Standard Protocols