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RALY Monoclonal antibody, PBS Only

RALY Monoclonal Antibody for WB, IHC, IF/ICC, FC (Intra), Indirect ELISA

Host / Isotype

Mouse / IgG1

Reactivity

human

Applications

WB, IHC, IF/ICC, FC (Intra), Indirect ELISA

Conjugate

Unconjugated

CloneNo.

2B10C3

Cat no : 68011-1-PBS

Print datasheet

Synonyms

HNRPCL2, hnRNP core protein C-like 2, hnRNP associated with lethal yellow protein homolog, Heterogeneous nuclear ribonucleoprotein C-like 2, Autoantigen p542

验证数据展示

  • WB analysis of LNCaP using 68011-1-Ig (same clone as 68011-1-PBS)

    WB analysis of LNCaP using 68011-1-Ig (same clone as 68011-1-PBS)

    LNCaP cells were subjected to SDS PAGE followed by western blot with 68011-1-Ig (RALY antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours. The membrane was stripped and reblotted with Lamin B1 Monoclonal antibody (66095-1-Ig) as loading control. This data was developed using the same antibody clone with 68011-1-PBS in a different storage buffer formulation.

  • IHC staining of human stomach cancer using 68011-1-Ig (same clone as 68011-1-PBS)

    IHC staining of human stomach cancer using 68011-1-Ig (same clone as 68011-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human stomach cancer tissue slide using 68011-1-Ig (RALY antibody) at dilution of 1:2000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 68011-1-PBS in a different storage buffer formulation.

  • IHC staining of human stomach cancer using 68011-1-Ig (same clone as 68011-1-PBS)

    IHC staining of human stomach cancer using 68011-1-Ig (same clone as 68011-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human stomach cancer tissue slide using 68011-1-Ig (RALY antibody) at dilution of 1:2000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 68011-1-PBS in a different storage buffer formulation.

  • IF Staining of A431 using 68011-1-Ig (same clone as 68011-1-PBS)

    IF Staining of A431 using 68011-1-Ig (same clone as 68011-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed A431 cells using RALY antibody (68011-1-Ig, Clone: 2B10C3 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 68011-1-PBS in a different storage buffer formulation.

  • FC experiment of A431 using 68011-1-Ig (same clone as 68011-1-PBS)

    FC experiment of A431 using 68011-1-Ig (same clone as 68011-1-PBS)

    1X10^6 A431 cells were intracellularly stained with 0.4 ug Anti-Human RALY (68011-1-Ig, Clone:2B10C3) and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L) at dilution 1:1000 (red), or 0.4 ug Mouse IgG1 Isotype Control (MOPC-21) (65124-1-Ig, Clone: MOPC-21) (blue). Cells were fixed and permeabilized with Transcription Factor Staining Buffer Kit (PF00011). This data was developed using the same antibody clone with 68011-1-PBS in a different storage buffer formulation.

产品信息

68011-1-PBS targets RALY in WB, IHC, IF/ICC, FC (Intra), Indirect ELISA applications and shows reactivity with human samples.

Tested Applications WB, IHC, IF/ICC, FC (Intra), Indirect ELISA Application Description
Tested Reactivity human
Immunogen RALY fusion protein Ag12250 种属同源性预测
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Full Name RNA binding protein, autoantigenic (hnRNP-associated with lethal yellow homolog (mouse))
Synonyms HNRPCL2, hnRNP core protein C-like 2, hnRNP associated with lethal yellow protein homolog, Heterogeneous nuclear ribonucleoprotein C-like 2, Autoantigen p542
Calculated Molecular Weight 307 aa, 33 kDa
Observed Molecular Weight30-37 kDa
GenBank Accession NumberBC103753
Gene Symbol RALY
Gene ID (NCBI) 22913
RRIDAB_2918758
Conjugate Unconjugated
Form Liquid
Purification MethodProtein G purification
UNIPROT IDQ9UKM9
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

RNA-binding protein RALY is a protein that in humans is encoded by the RALY gene. In infectious mononucleosis, anti-EBNA-1 antibodies are produced which cross-react with multiple normal human proteins. The cross-reactivity is due to anti-gly/ala antibodies that cross-react with host proteins containing configurations like those in the EBNA-1 repeat. One such antigen is RALY which is a member of the heterogeneous nuclear ribonucleoprotein gene family.