验证数据展示
经过测试的应用
| Positive WB detected in | LNCaP cells, HeLa cells, HepG2 cells, Jurkat cells, K-562 cells |
| Positive IHC detected in | human stomach cancer tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF/ICC detected in | A431 cells |
| Positive FC (Intra) detected in | A431 cells |
Planning an IF experiment? We recommend our CoraLite® Plus 488 and CoraLite®594 conjugated versions of this antibody.
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Western Blot (WB) | WB : 1:5000-1:50000 |
| Immunohistochemistry (IHC) | IHC : 1:1000-1:4000 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:200-1:800 |
| Flow Cytometry (FC) (INTRA) | FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
发表文章中的应用
| KD/KO | See 1 publications below |
| WB | See 1 publications below |
| CoIP | See 1 publications below |
产品信息
68011-1-Ig targets RALY in WB, IHC, IF/ICC, FC (Intra), CoIP, ELISA applications and shows reactivity with human samples.
| 经测试应用 | WB, IHC, IF/ICC, FC (Intra), ELISA Application Description |
| 文献引用应用 | WB, CoIP |
| 经测试反应性 | human |
| 文献引用反应性 | human |
| 免疫原 | RALY fusion protein Ag12250 种属同源性预测 |
| 宿主/亚型 | Mouse / IgG1 |
| 抗体类别 | Monoclonal |
| 产品类型 | Antibody |
| 全称 | RNA binding protein, autoantigenic (hnRNP-associated with lethal yellow homolog (mouse)) |
| 别名 | HNRPCL2, hnRNP core protein C-like 2, hnRNP associated with lethal yellow protein homolog, Heterogeneous nuclear ribonucleoprotein C-like 2, Autoantigen p542 |
| 计算分子量 | 307 aa, 33 kDa |
| 观测分子量 | 30-37 kDa |
| GenBank蛋白编号 | BC103753 |
| 基因名称 | RALY |
| Gene ID (NCBI) | 22913 |
| RRID | AB_2918758 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Protein G purification |
| UNIPROT ID | Q9UKM9 |
| 储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol , pH 7.3 |
| 储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
RNA-binding protein RALY is a protein that in humans is encoded by the RALY gene. In infectious mononucleosis, anti-EBNA-1 antibodies are produced which cross-react with multiple normal human proteins. The cross-reactivity is due to anti-gly/ala antibodies that cross-react with host proteins containing configurations like those in the EBNA-1 repeat. One such antigen is RALY which is a member of the heterogeneous nuclear ribonucleoprotein gene family.
实验方案
| Product Specific Protocols | |
|---|---|
| WB protocol for RALY antibody 68011-1-Ig | Download protocol |
| IHC protocol for RALY antibody 68011-1-Ig | Download protocol |
| IF protocol for RALY antibody 68011-1-Ig | Download protocol |
| FC protocol for RALY antibody 68011-1-Ig | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |