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验证数据展示

  • IF Staining of A431 using CL488-68011

    IF Staining of A431 using CL488-68011

    Immunofluorescent analysis of (4% PFA) fixed A431 cells using CoraLite® Plus 488 RALY antibody (CL488-68011, Clone: 2B10C3 ) at dilution of 1:600.

  • FC experiment of A431 using CL488-68011

    FC experiment of A431 using CL488-68011

    1X10^6 A431 cells were intracellularly stained with 0.4 ug CoraLite® Plus 488 Anti-Human RALY (CL488-68011, Clone:2B10C3) (red), or 0.4 ug Control Antibody. Cells were fixed and permeabilized with Transcription Factor Staining Buffer Kit (PF00011).

  • FC experiment of A431 using CL488-68011

    FC experiment of A431 using CL488-68011

    1X10^6 A431 cells were intracellularly stained with 0.4 ug CoraLite® Plus 488 Anti-Human RALY (CL488-68011, Clone:2B10C3) (red), or 0.4 ug CoraLite® Plus 488 Mouse IgG1 Isotype Control (MOPC-21) (CL488-65124, Clone: MOPC-21) (blue). Cells were fixed and permeabilized with Transcription Factor Staining Buffer Kit (PF00011).

CoraLite® Plus 488-conjugated RALY Monoclonal antibody

RALY Monoclonal Antibody for IF/ICC, FC (Intra)
Cat No. CL488-68011

产品说明书

CloneNo. 2B10C3

宿主/亚型

Mouse / IgG1

种属反应性

human

应用

IF/ICC, FC (Intra)

别名

HNRPCL2, hnRNP core protein C-like 2, hnRNP associated with lethal yellow protein homolog, Heterogeneous nuclear ribonucleoprotein C-like 2, Autoantigen p542

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  CoraLite® Plus 488
规格: 

-/ -

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经过测试的应用

Positive IF/ICC detected inA431 cells
Positive FC (Intra) detected inA431 cells
For other applications, we recommend the unconjugated version of this antibody, 68011-1-Ig

推荐稀释比

应用推荐稀释比
Immunofluorescence (IF)/ICCIF/ICC : 1:300-1:1200
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

CL488-68011 targets RALY in IF/ICC, FC (Intra) applications and shows reactivity with human samples.

经测试应用 IF/ICC, FC (Intra) Application Description
经测试反应性 human
免疫原 RALY fusion protein Ag12250 种属同源性预测
宿主/亚型 Mouse / IgG1
抗体类别 Monoclonal
产品类型 Antibody
全称 RNA binding protein, autoantigenic (hnRNP-associated with lethal yellow homolog (mouse))
别名 HNRPCL2, hnRNP core protein C-like 2, hnRNP associated with lethal yellow protein homolog, Heterogeneous nuclear ribonucleoprotein C-like 2, Autoantigen p542
计算分子量 307 aa, 33 kDa
观测分子量37 kDa
GenBank蛋白编号BC103753
基因名称 RALY
Gene ID (NCBI) 22913
RRIDAB_2934609
偶联类型 CoraLite® Plus 488 Fluorescent Dye
最大激发/发射波长493 nm / 522 nm
形式 Liquid
纯化方式Protein G purification
UNIPROT IDQ9UKM9
储存缓冲液 PBS with 50% glycerol, 0.05% Proclin300, 0.5% BSA , pH 7.3
储存条件Store at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

RNA-binding protein RALY is a protein that in humans is encoded by the RALY gene. In infectious mononucleosis, anti-EBNA-1 antibodies are produced which cross-react with multiple normal human proteins. The cross-reactivity is due to anti-gly/ala antibodies that cross-react with host proteins containing configurations like those in the EBNA-1 repeat. One such antigen is RALY which is a member of the heterogeneous nuclear ribonucleoprotein gene family.

实验方案

Product Specific Protocols
IF protocol for CL Plus 488 RALY antibody CL488-68011Download protocol
FC protocol for CL Plus 488 RALY antibody CL488-68011Download protocol
Standard Protocols
Click here to view our Standard Protocols