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CoraLite®594-conjugated RALY Monoclonal antibody

RALY Monoclonal Antibody for IF/ICC, FC (Intra)

Host / Isotype

Mouse / IgG1

Reactivity

Human

Applications

IF/ICC, FC (Intra)

Conjugate

CoraLite®594 Fluorescent Dye

CloneNo.

2B10C3

Cat no : CL594-68011

Print datasheet

Synonyms

HNRPCL2, hnRNP core protein C-like 2, hnRNP associated with lethal yellow protein homolog, Heterogeneous nuclear ribonucleoprotein C-like 2, Autoantigen p542

验证数据展示

  • IF Staining of A431 using CL594-68011

    IF Staining of A431 using CL594-68011

    Immunofluorescent analysis of (4% PFA) fixed A431 cells using CoraLite®594 RALY antibody (CL594-68011, Clone: 2B10C3 ) at dilution of 1:200.

  • FC experiment of A431 using CL594-68011

    FC experiment of A431 using CL594-68011

    1X10^6 A431 cells were intracellularly stained with 0.4 ug CoraLite®594 Anti-Human RALY (CL594-68011, Clone:2B10C3) (red), or 0.4 ug CoraLite®594 Mouse IgG1 Isotype Control (MOPC-21) (CL594-65124, Clone: MOPC-21) (blue). Cells were fixed and permeabilized with Transcription Factor Staining Buffer Kit (PF00011).

经过测试的应用

Positive IF/ICC detected inA431 cells
Positive FC (Intra) detected inA431 cells
For other applications, we recommend the unconjugated version of this antibody, 68011-1-Ig

推荐稀释比

ApplicationDilution
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

CL594-68011 targets RALY in IF/ICC, FC (Intra) applications and shows reactivity with Human samples.

Tested Applications IF/ICC, FC (Intra) Application Description
Tested Reactivity Human
Immunogen RALY fusion protein Ag12250 种属同源性预测
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Full Name RNA binding protein, autoantigenic (hnRNP-associated with lethal yellow homolog (mouse))
Synonyms HNRPCL2, hnRNP core protein C-like 2, hnRNP associated with lethal yellow protein homolog, Heterogeneous nuclear ribonucleoprotein C-like 2, Autoantigen p542
Calculated Molecular Weight 307 aa, 33 kDa
Observed Molecular Weight37 kDa
GenBank Accession NumberBC103753
Gene Symbol RALY
Gene ID (NCBI) 22913
RRIDAB_3084792
Conjugate CoraLite®594 Fluorescent Dye
Excitation/Emission Maxima Wavelengths588 nm / 604 nm
Form Liquid
Purification MethodProtein G purification
UNIPROT IDQ9UKM9
Storage Buffer PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
Storage ConditionsStore at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

RNA-binding protein RALY is a protein that in humans is encoded by the RALY gene. In infectious mononucleosis, anti-EBNA-1 antibodies are produced which cross-react with multiple normal human proteins. The cross-reactivity is due to anti-gly/ala antibodies that cross-react with host proteins containing configurations like those in the EBNA-1 repeat. One such antigen is RALY which is a member of the heterogeneous nuclear ribonucleoprotein gene family.

实验方案

Product Specific Protocols
IF protocol for CL594 RALY antibody CL594-68011Download protocol
FC protocol for CL594 RALY antibody CL594-68011Download protocol
Standard Protocols
Click here to view our Standard Protocols