• All
  • Primary Antibodies
  • Conjugated Antibodies for IF
  • Conjugated Antibodies for FC
  • Secondary Antibodies
  • Antibody Labeling KitsNew
  • ELISA Kits
  • IHC Kits
  • Magnetic Cell Separation Kits
  • Cytokines & Growth Factors
  • Neutralizing/activating Antibodies
  • Nanobody-based Reagents
  • Accessory Products and Kits
  • Fusion Proteins
×
×
Host
0
Species Reactivity
0
Species Reactivity
0
Conjugate
0
Conjugate
0
Compatible Species
0
Conjugate
0

RAN Recombinant antibody, PBS Only

RAN Recombinant Antibody for WB,Indirect ELISA,IF,FC

Host / Isotype

Rabbit / IgG

Reactivity

Human, mouse, rat

Applications

WB,Indirect ELISA,IF,FC

Conjugate

Unconjugated

CloneNo.

230317F3

Cat No : 82978-1-PBS

Print datasheet

Synonyms

ARA24, Gsp1, GTPase Ran, RAN, Ras like protein TC4, Ras related nuclear protein, TC4

验证数据展示

  • WB analysis using 82978-1-RR (same clone as 82978-1-PBS)

    WB analysis using 82978-1-RR (same clone as 82978-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 82978-1-RR (RAN antibody) at dilution of 1:2500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 82978-1-PBS in a different storage buffer formulation.

  • FC experiment of A431 using 82978-1-RR (same clone as 82978-1-PBS)

    FC experiment of A431 using 82978-1-RR (same clone as 82978-1-PBS)

    1x10^6 A431 cells were intracellularly stained with 0.25 ug Anti-Human RAN (82978-1-RR, Clone:230317F3) and APC-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L)(red), or 0.25 ug Rabbit IgG control Rabbit PolyAb (30000-0-AP) (blue). Cells were fixed and permeabilized with True-Nuclear Transcription Factor Buffer Set. This data was developed using the same antibody clone with 82978-1-PBS in a different storage buffer formulation.

  • FC experiment of HeLa using 82978-1-RR (same clone as 82978-1-PBS)

    FC experiment of HeLa using 82978-1-RR (same clone as 82978-1-PBS)

    1x10^6 HeLa cells were intracellularly stained with 0.25 ug Anti-Human RAN (82978-1-RR, Clone:230317F3) and APC-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L)(red), or 0.25 ug Rabbit IgG control Rabbit PolyAb (30000-0-AP) (blue). Cells were fixed and permeabilized with True-Nuclear Transcription Factor Buffer Set. This data was developed using the same antibody clone with 82978-1-PBS in a different storage buffer formulation.

  • IF Staining of HeLa using 82978-1-RR (same clone as 82978-1-PBS)

    IF Staining of HeLa using 82978-1-RR (same clone as 82978-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed HeLa cells using RAN antibody (82978-1-RR, Clone: 230317F3 ) at dilution of 1:100 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L) (SA00013-1). This data was developed using the same antibody clone with 82978-1-PBS in a different storage buffer formulation.

产品信息

82978-1-PBS targets RAN in WB, IF, FC, Indirect ELISA applications and shows reactivity with Human, mouse, rat samples.

Tested Applications WB,Indirect ELISA,IF,FC Application Description
Tested Reactivity Human, mouse, rat
Immunogen RAN fusion protein Ag0748 种属同源性预测
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Full Name RAN, member RAS oncogene family
Synonyms ARA24, Gsp1, GTPase Ran, RAN, Ras like protein TC4, Ras related nuclear protein, TC4
Calculated Molecular Weight 25 kDa
Observed Molecular Weight 24 kDa
GenBank Accession NumberBC004272
Gene Symbol RAN
Gene ID (NCBI) 5901
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
UNIPROT IDP62826
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.

背景介绍

RAN is also named as ARA24, Ras-like protein TC4 and GTPase Ran, and belongs to the small GTPase superfamily family and the Ran family. GTPase Ran switches between a cytoplasmic GDP- and a nuclear GTP-bound state by nucleotide exchange and GTP hydrolysis (PMID:7819259, PMID:32528950). RAN (GTP-bound form) triggers microtubule assembly at mitotic chromosomes and is required for normal mitotic spindle assembly and chromosome segregation (PMID:10408446, PMID:29040603, PMID:32528950).