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RAN Recombinant antibody

RAN Uni-rAbTM Recombinant Antibody for WB, IHC, IF/ICC, FC (Intra), ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, FC (Intra), ELISA

Conjugate

Unconjugated

CloneNo.

230317F3

Cat no : 82978-1-RR

Print datasheet

Synonyms

ARA24, GTPase Ran, GTP binding nuclear protein Ran, EC:3.6.5.-, Androgen receptor-associated protein 24

验证数据展示

  • WB analysis using 82978-1-RR

    WB analysis using 82978-1-RR

    Various lysates were subjected to SDS PAGE followed by western blot with 82978-1-RR (RAN antibody) at dilution of 1:2500 incubated at room temperature for 1.5 hours.

  • FC experiment of A431 using 82978-1-RR

    FC experiment of A431 using 82978-1-RR

    1x10^6 A431 cells were intracellularly stained with 0.25 ug Anti-Human RAN (82978-1-RR, Clone:230317F3) and APC-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L)(red), or 0.25 ug Rabbit IgG control Rabbit PolyAb (30000-0-AP) (blue). Cells were fixed and permeabilized with True-Nuclear Transcription Factor Buffer Set.

  • FC experiment of HeLa using 82978-1-RR

    FC experiment of HeLa using 82978-1-RR

    1x10^6 HeLa cells were intracellularly stained with 0.25 ug Anti-Human RAN (82978-1-RR, Clone:230317F3) and APC-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L)(red), or 0.25 ug Rabbit IgG control Rabbit PolyAb (30000-0-AP) (blue). Cells were fixed and permeabilized with True-Nuclear Transcription Factor Buffer Set.

  • IHC staining of human colon using 82978-1-RR

    IHC staining of human colon using 82978-1-RR

    Immunohistochemical analysis of paraffin-embedded human colon tissue slide using 82978-1-RR (RAN antibody) at dilution of 1:500 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with RAN antibody included
  • IHC staining of rat kidney using 82978-1-RR

    IHC staining of rat kidney using 82978-1-RR

    Immunohistochemical analysis of paraffin-embedded rat kidney tissue slide using 82978-1-RR (RAN antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with RAN antibody included
  • IHC staining of human placenta using 82978-1-RR

    IHC staining of human placenta using 82978-1-RR

    Immunohistochemical analysis of paraffin-embedded human placenta tissue slide using 82978-1-RR (RAN antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with RAN antibody included
  • IF Staining of HeLa using 82978-1-RR

    IF Staining of HeLa using 82978-1-RR

    Immunofluorescent analysis of (4% PFA) fixed HeLa cells using RAN antibody (82978-1-RR, Clone: 230317F3 ) at dilution of 1:250 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red).

  • IHC staining of mouse kidney using 82978-1-RR

    IHC staining of mouse kidney using 82978-1-RR

    Immunohistochemical analysis of paraffin-embedded mouse kidney tissue slide using 82978-1-RR (RAN antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with RAN antibody included
  • Affinity and Kinetic Characterization of 82978-1-RR

    Affinity and Kinetic Characterization of 82978-1-RR

    Biolayer interferometry (BLl) kinetic assays of 82978-1-RR against Human RAN were performed. The affinity constant is 3.08 nM.

经过测试的应用

Positive WB detected inHeLa cells, A375 cells, Jurkat cells, NIH/3T3 cells, C6 cells
Positive IHC detected inhuman colon tissue, mouse kidney tissue, rat kidney tissue, human placenta tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inHeLa cells
Positive FC (Intra) detected inHeLa cells, A431 cells
Planning an IHC experiment? We recommend our IHCeasy RAN Ready-To-Use IHC Kit. RAN primary antibody included.

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:5000
Immunohistochemistry (IHC)IHC : 1:250-1:1000
Immunofluorescence (IF)/ICCIF/ICC : 1:125-1:500
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.25 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

82978-1-RR targets RAN in WB, IHC, IF/ICC, FC (Intra), ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Applications WB, IHC, IF/ICC, FC (Intra), ELISA Application Description
Tested Reactivity human, mouse, rat
Immunogen RAN fusion protein Ag0748 种属同源性预测
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Full Name RAN, member RAS oncogene family
Synonyms ARA24, GTPase Ran, GTP binding nuclear protein Ran, EC:3.6.5.-, Androgen receptor-associated protein 24
Calculated Molecular Weight 25 kDa
Observed Molecular Weight 24 kDa
GenBank Accession NumberBC004272
Gene Symbol RAN
Gene ID (NCBI) 5901
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
UNIPROT IDP62826
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

RAN is also named as ARA24, Ras-like protein TC4 and GTPase Ran, and belongs to the small GTPase superfamily family and the Ran family. GTPase Ran switches between a cytoplasmic GDP- and a nuclear GTP-bound state by nucleotide exchange and GTP hydrolysis (PMID:7819259, PMID:32528950). RAN (GTP-bound form) triggers microtubule assembly at mitotic chromosomes and is required for normal mitotic spindle assembly and chromosome segregation (PMID:10408446, PMID:29040603, PMID:32528950).

实验方案

Product Specific Protocols
WB protocol for RAN antibody 82978-1-RRDownload protocol
IHC protocol for RAN antibody 82978-1-RRDownload protocol
IF protocol for RAN antibody 82978-1-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols