验证数据展示
经过测试的应用
| Positive IF/ICC detected in | HeLa cells |
Planning an IHC experiment? We recommend our IHCeasy RAN Ready-To-Use IHC Kit. RAN primary antibody included.
For other applications, we recommend the unconjugated version of this antibody, 82978-1-PBS
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Immunofluorescence (IF)/ICC | IF/ICC : 1:50-1:500 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
CL488-82978 targets RAN in IF/ICC applications and shows reactivity with human, mouse, rat samples.
| 经测试应用 | IF/ICC Application Description |
| 经测试反应性 | human, mouse, rat |
| 免疫原 | RAN fusion protein Ag0748 种属同源性预测 |
| 宿主/亚型 | Rabbit / IgG |
| 抗体类别 | Recombinant |
| 产品类型 | Antibody |
| 全称 | RAN, member RAS oncogene family |
| 别名 | ARA24, GTPase Ran, GTP binding nuclear protein Ran, EC:3.6.5.-, Androgen receptor-associated protein 24 |
| 计算分子量 | 25 kDa |
| 观测分子量 | 24 kDa |
| GenBank蛋白编号 | BC004272 |
| 基因名称 | RAN |
| Gene ID (NCBI) | 5901 |
| RRID | AB_3673162 |
| 偶联类型 | CoraLite® Plus 488 Fluorescent Dye |
| 最大激发/发射波长 | 493 nm / 522 nm |
| 形式 | Liquid |
| 纯化方式 | Protein A purification |
| UNIPROT ID | P62826 |
| 储存缓冲液 | PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3. |
| 储存条件 | Store at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
RAN is also named as ARA24, Ras-like protein TC4 and GTPase Ran, and belongs to the small GTPase superfamily family and the Ran family. GTPase Ran switches between a cytoplasmic GDP- and a nuclear GTP-bound state by nucleotide exchange and GTP hydrolysis (PMID:7819259, PMID:32528950). RAN (GTP-bound form) triggers microtubule assembly at mitotic chromosomes and is required for normal mitotic spindle assembly and chromosome segregation (PMID:10408446, PMID:29040603, PMID:32528950).
实验方案
| Product Specific Protocols | |
|---|---|
| IF protocol for CL Plus 488 RAN antibody CL488-82978 | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |