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验证数据展示

  • FC experiment of HeLa using CL594-67500

    FC experiment of HeLa using CL594-67500

    1X10^6 HeLa cells were intracellularly stained with 0.4 ug CoraLite®594 Anti-Human RAN (CL594-67500, Clone:1B11E1) (red), or 0.4 ug Control Antibody. Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

CoraLite®594-conjugated RAN Monoclonal antibody

RAN Monoclonal Antibody for FC (Intra)
Cat No. CL594-67500

产品说明书

CloneNo. 1B11E1

宿主/亚型

Mouse / IgG2b

种属反应性

human

应用

FC (Intra)

别名

ARA24, GTPase Ran, GTP binding nuclear protein Ran, EC:3.6.5.-, Androgen receptor-associated protein 24

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  CoraLite®594
规格: 

-/ -

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经过测试的应用

Positive FC (Intra) detected inHeLa cells
Planning an IHC experiment? We recommend our IHCeasy RAN Ready-To-Use IHC Kit. RAN primary antibody included.
For other applications, we recommend the unconjugated version of this antibody, 67500-1-Ig

推荐稀释比

应用推荐稀释比
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

CL594-67500 targets RAN in FC (Intra) applications and shows reactivity with human samples.

经测试应用 FC (Intra) Application Description
经测试反应性 human
免疫原 RAN fusion protein Ag30034 种属同源性预测
宿主/亚型 Mouse / IgG2b
抗体类别 Monoclonal
产品类型 Antibody
全称 RAN, member RAS oncogene family
别名 ARA24, GTPase Ran, GTP binding nuclear protein Ran, EC:3.6.5.-, Androgen receptor-associated protein 24
计算分子量 25 kDa
观测分子量 24 kDa
GenBank蛋白编号BC004272
基因名称 RAN
Gene ID (NCBI) 5901
RRIDAB_2934770
偶联类型 CoraLite®594 Fluorescent Dye
最大激发/发射波长588 nm / 604 nm
形式 Liquid
纯化方式Protein A purification
UNIPROT IDP62826
储存缓冲液 PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
储存条件Store at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

RAN is also named as ARA24, Ras-like protein TC4 and GTPase Ran, and belongs to the small GTPase superfamily family and the Ran family. GTPase Ran switches between a cytoplasmic GDP- and a nuclear GTP-bound state by nucleotide exchange and GTP hydrolysis (PMID:7819259, PMID:32528950). RAN (GTP-bound form) triggers microtubule assembly at mitotic chromosomes and is required for normal mitotic spindle assembly and chromosome segregation (PMID:10408446, PMID:29040603, PMID:32528950).

实验方案

Product Specific Protocols
FC protocol for CL594 RAN antibody CL594-67500Download protocol
Standard Protocols
Click here to view our Standard Protocols