CoraLite® Plus 594-conjugated RANGAP1 Monoclonal antibody
RANGAP1 Monoclonal Antibody for IF/ICC
Host / Isotype
Mouse / IgG1
Reactivity
Human, mouse, rat
Applications
IF/ICC
Conjugate
CoraLite® Plus 594 Fluorescent Dye
CloneNo.
1F3A4
验证数据展示
经过测试的应用
Positive IF detected in | HepG2 cells |
67146-1-Ig
For other applications, we recommend the unconjugated version of this antibody, 67146-1-Ig
推荐稀释比
Application | Dilution |
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Immunofluorescence (IF) | IF : 1:500-1:2000 |
It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
Sample-dependent, Check data in validation data gallery. |
产品信息
CL594-67146 targets RANGAP1 in IF applications and shows reactivity with Human, mouse, rat samples.
Tested Applications | IF/ICC Application Description |
Tested Reactivity | Human, mouse, rat |
Immunogen | RANGAP1 fusion protein Ag26136 种属同源性预测 |
Host / Isotype | Mouse / IgG1 |
Class | Monoclonal |
Type | Antibody |
Full Name | Ran GTPase activating protein 1 |
Synonyms | Fug1, KIAA1835, RANGAP1, SD |
Calculated Molecular Weight | 64 kDa |
Observed Molecular Weight | 64 kDa, 82 kDa |
GenBank Accession Number | BC014044 |
Gene Symbol | RANGAP1 |
Gene ID (NCBI) | 5905 |
Conjugate | CoraLite® Plus 594 Fluorescent Dye |
Excitation/Emission Maxima Wavelengths | 594 nm / 615 nm |
Form | Liquid |
Purification Method | Protein G purification |
UNIPROT ID | P46060 |
Storage Buffer | PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3. |
Storage Conditions | Store at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
RanGAP1 is a protein that associates with the nuclear pore complex and participates in the regulation of nuclear transport. In mammalian cells, unmodified RanGAP1 is predominantly cytoplasmic, whereas modification by small ubiquitin-related modifier protein (SUMO) targets RanGAP1 to the cytoplasmic filaments of nuclear pore complex (NPC) where it forms a complex with RanBP2 and Ubc9. Phosphorylation of RanGAP1 occurs in a cell-cycle-dependent manner and may play a role in regulating RanGAP1 catalytic activity. We got 64 kDa (cytoplasmic Ran GAP1) and 82 kDa (SUMO-1 modified Ran GAP1) in western blotting.
实验方案
Product Specific Protocols | |
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IF protocol for CL Plus 594 RANGAP1 antibody CL594-67146 | Download protocol |
Standard Protocols | |
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Click here to view our Standard Protocols |