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RAP1A Recombinant antibody, PBS Only

RAP1A Uni-rAbTM Recombinant Antibody for ELISA, FC (Intra), WB

Host / Isotype

Rabbit / IgG

Reactivity

rat, mouse, human

Applications

ELISA, FC (Intra), WB

Conjugate

Unconjugated

CloneNo.

240438E10

Cat no : 83708-2-PBS

Print datasheet

Synonyms

Ras-related protein Krev-1, KREV1, GTP-binding protein smg p21A, G-22K, EC:3.6.5.2

验证数据展示

  • WB analysis using 83708-2-RR (same clone as 83708-2-PBS)

    WB analysis using 83708-2-RR (same clone as 83708-2-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 83708-2-RR (RAP1A antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 83708-2-PBS in a different storage buffer formulation.

  • FC experiment of HeLa using 83708-2-RR (same clone as 83708-2-PBS)

    FC experiment of HeLa using 83708-2-RR (same clone as 83708-2-PBS)

    1x10^6 HeLa cells were intracellularly stained with 0.25 ug RAP1A Recombinant antibody (83708-2-RR, Clone:240438E10) and APC-Conjugated Goat Anti-Rabbit IgG(H+L)(red), or 0.25 ug Isotype Control (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C). This data was developed using the same antibody clone with 83708-2-PBS in a different storage buffer formulation.

  • Affinity and Kinetic Characterization of 83708-2-RR

    Affinity and Kinetic Characterization of 83708-2-RR

    Biolayer interferometry (BLl) kinetic assays of 83708-2-RR against Human RAP1A were performed. The affinity constant is 0.232 nM.

产品信息

The immunogen of 83708-2-PBS is RAP1A Fusion Protein expressed in E. coli.

Tested Applications ELISA, FC (Intra), WB Application Description
Tested Reactivity rat, mouse, human
Immunogen RAP1A fusion protein Ag9488 种属同源性预测
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Full Name RAP1A, member of RAS oncogene family
Synonyms Ras-related protein Krev-1, KREV1, GTP-binding protein smg p21A, G-22K, EC:3.6.5.2
Calculated Molecular Weight 184 aa, 21 kDa
GenBank Accession NumberBC014086
Gene Symbol RAP1A
Gene ID (NCBI) 5906
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purfication
UNIPROT IDP62834
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C