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RAP1A Recombinant antibody, PBS Only

RAP1A Uni-rAbTM Recombinant Antibody for ELISA, FC (Intra), WB

Host / Isotype

Rabbit / IgG

Reactivity

rat, mouse, human

Applications

ELISA, FC (Intra), WB

Conjugate

Unconjugated

CloneNo.

240438E10

Cat no : 83708-2-PBS

Print datasheet

Synonyms

Ras-related protein Krev-1, KREV1, GTP-binding protein smg p21A, G-22K, EC:3.6.5.2

验证数据展示

  • WB analysis using 83708-2-RR (same clone as 83708-2-PBS)

    WB analysis using 83708-2-RR (same clone as 83708-2-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 83708-2-RR (RAP1A antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 83708-2-PBS in a different storage buffer formulation.

  • FC experiment of HeLa using 83708-2-RR (same clone as 83708-2-PBS)

    FC experiment of HeLa using 83708-2-RR (same clone as 83708-2-PBS)

    1x10^6 HeLa cells were intracellularly stained with 0.25 ug RAP1A Recombinant antibody (83708-2-RR, Clone:240438E10) and APC-Conjugated Goat Anti-Rabbit IgG(H+L)(red), or 0.25 ug Isotype Control (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C). This data was developed using the same antibody clone with 83708-2-PBS in a different storage buffer formulation.

  • Affinity and Kinetic Characterization of 83708-2-RR

    Affinity and Kinetic Characterization of 83708-2-RR

    Biolayer interferometry (BLl) kinetic assays of 83708-2-RR against Human RAP1A were performed. The affinity constant is 0.232 nM.

产品信息

83708-2-PBS targets RAP1A in ELISA, FC (Intra), WB applications and shows reactivity with rat, mouse, human samples.

Tested Applications ELISA, FC (Intra), WB Application Description
Tested Reactivity rat, mouse, human
Immunogen RAP1A fusion protein Ag9488 种属同源性预测
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Full Name RAP1A, member of RAS oncogene family
Synonyms Ras-related protein Krev-1, KREV1, GTP-binding protein smg p21A, G-22K, EC:3.6.5.2
Calculated Molecular Weight 184 aa, 21 kDa
GenBank Accession NumberBC014086
Gene Symbol RAP1A
Gene ID (NCBI) 5906
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purfication
UNIPROT IDP62834
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C