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验证数据展示

  • WB analysis using 83708-2-RR

    WB analysis using 83708-2-RR

    Various lysates were subjected to SDS PAGE followed by western blot with 83708-2-RR (RAP1A antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

  • FC experiment of HeLa using 83708-2-RR

    FC experiment of HeLa using 83708-2-RR

    1x10^6 HeLa cells were intracellularly stained with 0.25 ug RAP1A Recombinant antibody (83708-2-RR, Clone:240438E10) and APC-Conjugated Goat Anti-Rabbit IgG(H+L)(red), or 0.25 ug Isotype Control (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

  • Affinity and Kinetic Characterization of 83708-2-RR

    Affinity and Kinetic Characterization of 83708-2-RR

    Biolayer interferometry (BLl) kinetic assays of 83708-2-RR against Human RAP1A were performed. The affinity constant is 0.232 nM.

RAP1A Recombinant antibody

RAP1A Uni-rAbTM Recombinant Antibody for WB, FC (Intra), ELISA
Cat No. 83708-2-RR

产品说明书

CloneNo. 240438E10

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse, rat

应用

WB, FC (Intra), ELISA

别名

Ras-related protein Krev-1, KREV1, GTP-binding protein smg p21A, G-22K, EC:3.6.5.2

缓冲液配方:  PBS and Azide
PBS and Azide
PBS Only
偶联物:  Unconjugated
Unconjugated
规格: 

-/ -

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经过测试的应用

Positive WB detected inNIH/3T3 cells, A431 cells, HeLa cells, K-562 cells, human platelet tissue, rat brain tissue
Positive FC (Intra) detected inHeLa cells

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:5000-1:50000
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.25 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

83708-2-RR targets RAP1A in WB, FC (Intra), ELISA applications and shows reactivity with human, mouse, rat samples.

经测试应用 WB, FC (Intra), ELISA Application Description
经测试反应性 human, mouse, rat
免疫原 RAP1A fusion protein Ag9488 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Recombinant
产品类型 Antibody
全称 RAP1A, member of RAS oncogene family
别名 Ras-related protein Krev-1, KREV1, GTP-binding protein smg p21A, G-22K, EC:3.6.5.2
计算分子量 184 aa, 21 kDa
观测分子量 21 kDa
GenBank蛋白编号BC014086
基因名称 RAP1A
Gene ID (NCBI) 5906
RRIDAB_3671311
偶联类型 Unconjugated
形式 Liquid
纯化方式Protein A purfication
UNIPROT IDP62834
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

RAP1A(Ras-related protein Rap-1A), also known as KREV1. Induces morphological reversion of a cell line transformed by a Ras oncogene. The biological roles of RAP1A include inducing neurite outgrowth and embryonic angiogenesis(PMID: 17916086). As one member of RAS oncogene family, it is a widely expressed receptor,with high mRNA expression in many tissues, particularly abundant in the colon. In cells, RAP1A was localized in cytoplasm and cell membrane(PMID: 11359771, PMID: 11359771).

实验方案

Product Specific Protocols
WB protocol for RAP1A antibody 83708-2-RRDownload protocol
FC protocol for RAP1A antibody 83708-2-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols