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验证数据展示

  • WB analysis using 67174-1-Ig

    WB analysis using 67174-1-Ig

    Various lysates were subjected to SDS PAGE followed by western blot with 67174-1-Ig (RAP1B antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

  • IHC staining of human oesophagus cancer using 67174-1-Ig

    IHC staining of human oesophagus cancer using 67174-1-Ig

    Immunohistochemical analysis of paraffin-embedded human oesophagus cancer tissue slide using 67174-1-Ig (RAP1B antibody) at dilution of 1:1 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with RAP1B antibody included
  • IHC staining of human oesophagus cancer using 67174-1-Ig

    IHC staining of human oesophagus cancer using 67174-1-Ig

    Immunohistochemical analysis of paraffin-embedded human oesophagus cancer tissue slide using 67174-1-Ig (RAP1B antibody) at dilution of 1:1 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with RAP1B antibody included
  • IF Staining of HeLa using 67174-1-Ig

    IF Staining of HeLa using 67174-1-Ig

    Immunofluorescent analysis of (-20°C Methanol) fixed HeLa cells using RAP1B antibody (67174-1-Ig, Clone: 2E7G11 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L).

RAP1B Monoclonal antibody

RAP1B Monoclonal Antibody for WB, IHC, IF/ICC, ELISA
Cat No. 67174-1-Ig

产品说明书

CloneNo. 2E7G11

宿主/亚型

Mouse / IgG1

种属反应性

Human, mouse, rat

应用

WB, IHC, IF/ICC, ELISA

别名

DKFZp586H0723, GTP binding protein smg p21B, K REV, RAL1B, RAP1B, Ras related protein Rap 1b

缓冲液配方:  PBS and Azide
PBS and Azide
PBS Only
偶联物:  Unconjugated
Unconjugated
CoraLite® Plus 488
规格: 

-/ -

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经过测试的应用

Positive WB detected inHeLa cells, HSC-T6 cells, NIH/3T3 cells, 4T1 cells, HEK-293 cells, HepG2 cells, Jurkat cells
Positive IHC detected inhuman oesophagus cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inHeLa cells
Planning an IHC experiment? We recommend our IHCeasy RAP1B Ready-To-Use IHC Kit. RAP1B primary antibody included.
Planning an IF experiment? We recommend our CoraLite® Plus 488 conjugated versions of this antibody.

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:2000-1:8000
Immunohistochemistry (IHC)IHC : 1:50-1:200
Immunofluorescence (IF)/ICCIF/ICC : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

67174-1-Ig targets RAP1B in WB, IHC, IF/ICC, ELISA applications and shows reactivity with Human, mouse, rat samples.

经测试应用 WB, IHC, IF/ICC, ELISA Application Description
经测试反应性 Human, mouse, rat
免疫原 RAP1B fusion protein Ag29212 种属同源性预测
宿主/亚型 Mouse / IgG1
抗体类别 Monoclonal
产品类型 Antibody
全称 RAP1B, member of RAS oncogene family
别名 DKFZp586H0723, GTP binding protein smg p21B, K REV, RAL1B, RAP1B, Ras related protein Rap 1b
计算分子量 21 kDa
观测分子量 21 kDa
GenBank蛋白编号BC000176
基因名称 RAP1B
Gene ID (NCBI) 5908
RRIDAB_2882470
偶联类型 Unconjugated
形式 Liquid
纯化方式Protein A purification
UNIPROT IDP61224
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

实验方案

Product Specific Protocols
WB protocol for RAP1B antibody 67174-1-IgDownload protocol
IHC protocol for RAP1B antibody 67174-1-IgDownload protocol
IF protocol for RAP1B antibody 67174-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols