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RAP1B Monoclonal antibody

RAP1B Monoclonal Antibody for WB, IF/ICC, IHC, ELISA

Host / Isotype

Mouse / IgG1

Reactivity

Human, mouse, rat

Applications

WB, IF/ICC, IHC, ELISA

Conjugate

Unconjugated

CloneNo.

2E7G11

Cat No : 67174-1-Ig

Print datasheet

Synonyms

DKFZp586H0723, GTP binding protein smg p21B, K REV, RAL1B, RAP1B, Ras related protein Rap 1b

验证数据展示

  • WB analysis using 67174-1-Ig

    WB analysis using 67174-1-Ig

    Various lysates were subjected to SDS PAGE followed by western blot with 67174-1-Ig (RAP1B antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

  • IHC staining of human oesophagus cancer using 67174-1-Ig

    IHC staining of human oesophagus cancer using 67174-1-Ig

    Immunohistochemical analysis of paraffin-embedded human oesophagus cancer tissue slide using 67174-1-Ig (RAP1B antibody) at dilution of 1:1 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with RAP1B antibody included
  • IHC staining of human oesophagus cancer using 67174-1-Ig

    IHC staining of human oesophagus cancer using 67174-1-Ig

    Immunohistochemical analysis of paraffin-embedded human oesophagus cancer tissue slide using 67174-1-Ig (RAP1B antibody) at dilution of 1:1 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with RAP1B antibody included
  • IF Staining of HeLa using 67174-1-Ig

    IF Staining of HeLa using 67174-1-Ig

    Immunofluorescent analysis of (-20°C Methanol) fixed HeLa cells using RAP1B antibody (67174-1-Ig, Clone: 2E7G11 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L).

经过测试的应用

Positive WB detected inHeLa cells, HSC-T6 cells, NIH/3T3 cells, 4T1 cells, HEK-293 cells, HepG2 cells, Jurkat cells
Positive IHC detected inhuman oesophagus cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inHeLa cells
Planning an IHC experiment? We recommend our IHCeasy RAP1B Ready-To-Use IHC Kit. RAP1B primary antibody included.
Planning an IF experiment? We recommend our CoraLite® Plus 488 conjugated versions of this antibody.

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:2000-1:8000
Immunohistochemistry (IHC)IHC : 1:50-1:200
Immunofluorescence (IF)IF : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

67174-1-Ig targets RAP1B in WB, IF, IHC, ELISA applications and shows reactivity with Human, mouse, rat samples.

Tested Applications WB, IF/ICC, IHC, ELISA Application Description
Tested Reactivity Human, mouse, rat
Immunogen RAP1B fusion protein Ag29212 种属同源性预测
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Full Name RAP1B, member of RAS oncogene family
Synonyms DKFZp586H0723, GTP binding protein smg p21B, K REV, RAL1B, RAP1B, Ras related protein Rap 1b
Calculated Molecular Weight 21 kDa
Observed Molecular Weight 21 kDa
GenBank Accession NumberBC000176
Gene Symbol RAP1B
Gene ID (NCBI) 5908
RRIDAB_2882470
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
UNIPROT IDP61224
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

实验方案

Product Specific Protocols
WB protocol for RAP1B antibody 67174-1-IgDownload protocol
IHC protocol for RAP1B antibody 67174-1-IgDownload protocol
IF protocol for RAP1B antibody 67174-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols