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验证数据展示

  • FC experiment of HeLa using CL488-67174

    FC experiment of HeLa using CL488-67174

    1x10^6 HeLa cells were intracellularly stained with 0.4 ug CoraLite® Plus 488-conjugated RAP1B Monoclonal antibody (CL488-67174, Clone:2E7G11)(red), or 0.4 ug CoraLite® Plus 488 Mouse IgG1 Isotype Control (1F8D3) (CL488-66360-1, Clone: 1F8D3) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

  • IF Staining of HeLa using CL488-67174

    IF Staining of HeLa using CL488-67174

    Immunofluorescent analysis of (-20°C Ethanol) fixed HeLa cells using CoraLite® Plus 488 RAP1B antibody (CL488-67174, Clone: 2E7G11 ) at dilution of 1:200.

CoraLite® Plus 488-conjugated RAP1B Monoclonal antibody

RAP1B Monoclonal Antibody for IF/ICC, FC (Intra)
Cat No. CL488-67174

Print datasheet

CloneNo. 2E7G11

Host / Isotype

Mouse / IgG1

Reactivity

human, mouse, rat

Applications

IF/ICC, FC (Intra)

Synonyms

RAL1B, K REV, GTP-binding protein smg p21B, GTP binding protein smg p21B, EC:3.6.5.2

Formulation:  PBS and Azide
PBS and Azide
Conjugate:  CoraLite® Plus 488
Size: 

-/ -

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经过测试的应用

Positive IF/ICC detected inHeLa cells
Positive FC (Intra) detected inHeLa cells
Planning an IHC experiment? We recommend our IHCeasy RAP1B Ready-To-Use IHC Kit. RAP1B primary antibody included.
For other applications, we recommend the unconjugated version of this antibody, 67174-1-Ig

推荐稀释比

ApplicationDilution
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

CL488-67174 targets RAP1B in IF/ICC, FC (Intra) applications and shows reactivity with human, mouse, rat samples.

Tested Applications IF/ICC, FC (Intra) Application Description
Tested Reactivity human, mouse, rat
Immunogen RAP1B fusion protein Ag29212 种属同源性预测
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Full Name RAP1B, member of RAS oncogene family
Synonyms RAL1B, K REV, GTP-binding protein smg p21B, GTP binding protein smg p21B, EC:3.6.5.2
Calculated Molecular Weight 21 kDa
Observed Molecular Weight 21 kDa
GenBank Accession NumberBC000176
Gene Symbol RAP1B
Gene ID (NCBI) 5908
RRIDAB_3672939
Conjugate CoraLite® Plus 488 Fluorescent Dye
Excitation/Emission Maxima Wavelengths493 nm / 522 nm
Form Liquid
Purification MethodProtein G purification
UNIPROT IDP61224
Storage Buffer PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
Storage ConditionsStore at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

RAP1B is a member of the RAS-like small GTP-binding protein superfamily. Members of this family regulate multiple cellular processes including cell adhesion and growth and differentiation. RAP1B localizes to cellular membranes and has been shown to regulate integrin-mediated cell signaling. It also plays a role in regulating outside-in signaling in platelets.

实验方案

Product Specific Protocols
IF protocol for CL Plus 488 RAP1B antibody CL488-67174Download protocol
FC protocol for CL Plus 488 RAP1B antibody CL488-67174Download protocol
Standard Protocols
Click here to view our Standard Protocols