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CoraLite® Plus 488-conjugated RAP1B Monoclonal antibody

RAP1B Monoclonal Antibody for IF/ICC

Host / Isotype

Mouse / IgG1

Reactivity

Human, mouse, rat

Applications

IF/ICC

Conjugate

CoraLite® Plus 488 Fluorescent Dye

CloneNo.

2E7G11

Cat No : CL488-67174

Print datasheet

Synonyms

DKFZp586H0723, GTP binding protein smg p21B, K REV, RAL1B, RAP1B, Ras related protein Rap 1b

验证数据展示

  • IF Staining of HeLa using CL488-67174

    IF Staining of HeLa using CL488-67174

    Immunofluorescent analysis of (-20°C Ethanol) fixed HeLa cells using CoraLite® Plus 488 RAP1B antibody (CL488-67174, Clone: 2E7G11 ) at dilution of 1:200.

经过测试的应用

Positive IF detected inHeLa cells
Planning an IHC experiment? We recommend our IHCeasy RAP1B Ready-To-Use IHC Kit. RAP1B primary antibody included.
For other applications, we recommend the unconjugated version of this antibody, 67174-1-Ig

推荐稀释比

ApplicationDilution
Immunofluorescence (IF)IF : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

CL488-67174 targets RAP1B in IF applications and shows reactivity with Human, mouse, rat samples.

Tested Applications IF/ICC Application Description
Tested Reactivity Human, mouse, rat
Immunogen RAP1B fusion protein Ag29212 种属同源性预测
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Full Name RAP1B, member of RAS oncogene family
Synonyms DKFZp586H0723, GTP binding protein smg p21B, K REV, RAL1B, RAP1B, Ras related protein Rap 1b
Calculated Molecular Weight 21 kDa
Observed Molecular Weight 21 kDa
GenBank Accession NumberBC000176
Gene Symbol RAP1B
Gene ID (NCBI) 5908
Conjugate CoraLite® Plus 488 Fluorescent Dye
Excitation/Emission Maxima Wavelengths493 nm / 522 nm
Form Liquid
Purification MethodProtein G purification
UNIPROT IDP61224
Storage Buffer PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
Storage ConditionsStore at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

RAP1B is a member of the RAS-like small GTP-binding protein superfamily. Members of this family regulate multiple cellular processes including cell adhesion and growth and differentiation. RAP1B localizes to cellular membranes and has been shown to regulate integrin-mediated cell signaling. It also plays a role in regulating outside-in signaling in platelets.

实验方案

Product Specific Protocols
IF protocol for CL Plus 488 RAP1B antibody CL488-67174Download protocol
Standard Protocols
Click here to view our Standard Protocols