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  • Featured Product
  • KD/KO Validated

RARB Polyclonal antibody, PBS Only

RARB Polyclonal Antibody for WB, IHC, IF/ICC, Indirect ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IHC, IF/ICC, Indirect ELISA

Conjugate

Unconjugated

Cat no : 14013-1-PBS

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Synonyms

验证数据展示

  • WB analysis using 14013-1-AP (same clone as 14013-1-PBS)

    WB analysis using 14013-1-AP (same clone as 14013-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 14013-1-AP (RARB antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 14013-1-PBS in a different storage buffer formulation.

  • IHC staining of mouse colon using 14013-1-AP (same clone as 14013-1-PBS)

    IHC staining of mouse colon using 14013-1-AP (same clone as 14013-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse colon tissue slide using 14013-1-AP (RARB antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 14013-1-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with RARB antibody included
  • IHC staining of mouse colon using 14013-1-AP (same clone as 14013-1-PBS)

    IHC staining of mouse colon using 14013-1-AP (same clone as 14013-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse colon tissue slide using 14013-1-AP (RARB antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 14013-1-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with RARB antibody included
  • IF Staining of MCF-7 using 14013-1-AP (same clone as 14013-1-PBS)

    IF Staining of MCF-7 using 14013-1-AP (same clone as 14013-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed MCF-7 cells using RARB antibody (14013-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L), CL594-phalloidin (red). This data was developed using the same antibody clone with 14013-1-PBS in a different storage buffer formulation.

Planning an IHC experiment? We recommend our IHCeasy RARB Ready-To-Use IHC Kit. RARB primary antibody included.

产品信息

14013-1-PBS targets RARB in WB, IHC, IF/ICC, Indirect ELISA applications and shows reactivity with human, mouse samples.

Tested Applications WB, IHC, IF/ICC, Indirect ELISA Application Description
Tested Reactivity human, mouse
Immunogen RARB fusion protein Ag5119 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name retinoic acid receptor, beta
Synonyms
Calculated Molecular Weight 50 kDa
Observed Molecular Weight37-51 kDa
GenBank Accession NumberBC060794
Gene Symbol RARB
Gene ID (NCBI) 5915
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDP10826
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

Retinoic acid receptors (RARs) are important mediators of retinoid signaling in morphogenesis, development, and cell differentiation. Retinoic acid receptors bind as heterodimers to their target response elements in response to their ligands, all-trans or 9-cis retinoic acid, and regulate gene expression in various biological processes (PMID:12554770). They can bind to the retinoic acid response elements (RARE) composed of tandem 5'-AGGTCA-3' sites known as DR1-DR5. In the absence or presence of hormone ligand, they act mainly as activators of gene expression due to weak binding to corepressors. RARB is one isotypes of RARs (PMID:12834868).