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验证数据展示

  • WB analysis using 12935-1-AP

    WB analysis using 12935-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 12935-1-AP (RASGAP antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours.

  • IP experiment of mouse testis using 12935-1-AP

    IP experiment of mouse testis using 12935-1-AP

    IP result of anti-RASGAP (IP:12935-1-AP, 4ug; Detection:12935-1-AP 1:4000) with mouse testis tissue lysate 1120 ug.

  • IHC staining of human testis using 12935-1-AP

    IHC staining of human testis using 12935-1-AP

    Immunohistochemical analysis of paraffin-embedded human testis tissue slide using 12935-1-AP (RASGAP antibody at dilution of 1:50 (under 10x lens).

    Ready-to-use IHC kits available with RASA1 antibody included
  • IHC staining of human testis using 12935-1-AP

    IHC staining of human testis using 12935-1-AP

    Immunohistochemical analysis of paraffin-embedded human testis tissue slide using 12935-1-AP (RASGAP antibody at dilution of 1:50 (under 40x lens).

    Ready-to-use IHC kits available with RASA1 antibody included
  • IF Staining of HeLa using 12935-1-AP

    IF Staining of HeLa using 12935-1-AP

    Immunofluorescent analysis of (-20°C Methanol) fixed HeLa cells using RASGAP antibody (12935-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L).

RASGAP Polyclonal antibody

RASGAP Polyclonal Antibody for WB, IHC, IF/ICC, IP, ELISA
Cat No. 12935-1-AP

产品说明书

引用文献(2)

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse, rat

应用

WB, IHC, IF/ICC, IP, ELISA

别名

RASA1, Ras p21 protein activator, Ras GTPase-activating protein 1, p120RASGAP, p120GAP

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  Unconjugated
Unconjugated
CoraLite® Plus 488
规格: 

-/ -

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经过测试的应用

Positive WB detected inmouse testis tissue, rat testis tissue
Positive IP detected inmouse testis tissue
Positive IHC detected inhuman testis tissue, human breast cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inHeLa cells
Planning an IHC experiment? We recommend our IHCeasy RASA1 Ready-To-Use IHC Kit. RASA1 primary antibody included.
Planning an IF experiment? We recommend our CoraLite® Plus 488 conjugated versions of this antibody.

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:1000-1:8000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:20-1:200
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

发表文章中的应用

WBSee 2 publications below

产品信息

12935-1-AP targets RASGAP in WB, IHC, IF/ICC, IP, ELISA applications and shows reactivity with human, mouse, rat samples.

经测试应用 WB, IHC, IF/ICC, IP, ELISA Application Description
文献引用应用WB
经测试反应性 human, mouse, rat
文献引用反应性human, rat
免疫原 RASGAP fusion protein Ag3906 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 RAS p21 protein activator (GTPase activating protein) 1
别名 RASA1, Ras p21 protein activator, Ras GTPase-activating protein 1, p120RASGAP, p120GAP
计算分子量 1047 aa, 116 kDa
观测分子量 120 kDa, 95 kDa
GenBank蛋白编号BC033015
基因名称 RASA1
Gene ID (NCBI) 5921
RRIDAB_2175906
偶联类型 Unconjugated
形式 Liquid
纯化方式Antigen affinity purification
UNIPROT IDP20936
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

实验方案

Product Specific Protocols
WB protocol for RASGAP antibody 12935-1-APDownload protocol
IHC protocol for RASGAP antibody 12935-1-APDownload protocol
IF protocol for RASGAP antibody 12935-1-APDownload protocol
IP protocol for RASGAP antibody 12935-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
ratWB

Biochem Biophys Res Commun

miR-335 is involved in the rat epididymal development by targeting the mRNA of RASA1.

Authors - Wang Junfeng J
View Article
humanWB

Int J Clin Exp Pathol

Quantitative proteomic analysis identifies new effectors of FOXM1 involved in breast cancer cell migration.

Authors - Xiaojuan Ye
View Article