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CoraLite® Plus 488-conjugated RASGAP Polyclonal antibody

RASGAP Polyclonal Antibody for IF/ICC

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

IF/ICC

Conjugate

CoraLite® Plus 488 Fluorescent Dye

Cat no : CL488-12935

Print datasheet

Synonyms

CM AVM, CMAVM, DKFZp434N071, GAP, GTPase activating protein, p120GAP, p120RASGAP, PKWS, Ras p21 protein activator, RASA, RASA1, RASGAP

验证数据展示

  • IF Staining of HeLa using CL488-12935

    IF Staining of HeLa using CL488-12935

    Immunofluorescent analysis of (-20°C Methanol) fixed HeLa cells using CoraLite® Plus 488 RASGAP antibody (CL488-12935) at dilution of 1:200.

经过测试的应用

Positive IF/ICC detected inHeLa cells
Planning an IHC experiment? We recommend our IHCeasy RASA1 Ready-To-Use IHC Kit. RASA1 primary antibody included.
For other applications, we recommend the unconjugated version of this antibody, 12935-1-AP

推荐稀释比

ApplicationDilution
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

CL488-12935 targets RASGAP in IF/ICC applications and shows reactivity with human, mouse, rat samples.

Tested Applications IF/ICC Application Description
Tested Reactivity human, mouse, rat
Immunogen RASGAP fusion protein Ag3906 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name RAS p21 protein activator (GTPase activating protein) 1
Synonyms CM AVM, CMAVM, DKFZp434N071, GAP, GTPase activating protein, p120GAP, p120RASGAP, PKWS, Ras p21 protein activator, RASA, RASA1, RASGAP
Calculated Molecular Weight 1047 aa, 116 kDa
Observed Molecular Weight 120 kDa, 95 kDa
GenBank Accession NumberBC033015
Gene Symbol RASA1
Gene ID (NCBI) 5921
Conjugate CoraLite® Plus 488 Fluorescent Dye
Excitation/Emission Maxima Wavelengths493 nm / 522 nm
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDP20936
Storage Buffer PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
Storage ConditionsStore at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

实验方案

Product Specific Protocols
IF protocol for CL Plus 488 RASGAP antibody CL488-12935Download protocol
Standard Protocols
Click here to view our Standard Protocols