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RASEF Monoclonal antibody, PBS Only

RASEF Monoclonal Antibody for WB, IHC, IF/ICC, Indirect ELISA

Host / Isotype

Mouse / IgG2a

Reactivity

Human, mouse

Applications

WB, IHC, IF/ICC, Indirect ELISA

Conjugate

Unconjugated

CloneNo.

1C2B2

Cat No : 67159-1-PBS

Print datasheet

Synonyms

FLJ31614, RAB45, Ras related protein Rab 45, RASEF

验证数据展示

  • WB analysis of LNCaP using 67159-1-Ig (same clone as 67159-1-PBS)

    WB analysis of LNCaP using 67159-1-Ig (same clone as 67159-1-PBS)

    LNCaP cells were subjected to SDS PAGE followed by western blot with 67159-1-Ig (RASEF antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67159-1-PBS in a different storage buffer formulation.

  • IHC staining of human prostate cancer using 67159-1-Ig (same clone as 67159-1-PBS)

    IHC staining of human prostate cancer using 67159-1-Ig (same clone as 67159-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human prostate cancer tissue slide using 67159-1-Ig (RASEF antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67159-1-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with RASEF antibody included
  • IF Staining of A431 using 67159-1-Ig (same clone as 67159-1-PBS)

    IF Staining of A431 using 67159-1-Ig (same clone as 67159-1-PBS)

    Immunofluorescent analysis of (-20°C Methanol) fixed A431 cells using RASEF antibody (67159-1-Ig, Clone: 1C2B2 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 67159-1-PBS in a different storage buffer formulation.

  • IHC staining of human prostate cancer using 67159-1-Ig (same clone as 67159-1-PBS)

    IHC staining of human prostate cancer using 67159-1-Ig (same clone as 67159-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human prostate cancer tissue slide using 67159-1-Ig (RASEF antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67159-1-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with RASEF antibody included
Planning an IHC experiment? We recommend our IHCeasy RASEF Ready-To-Use IHC Kit. RASEF primary antibody included.

产品信息

67159-1-PBS targets RASEF in WB, IHC, IF/ICC, Indirect ELISA applications and shows reactivity with Human, mouse samples.

Tested Applications WB, IHC, IF/ICC, Indirect ELISA Application Description
Tested Reactivity Human, mouse
Immunogen RASEF fusion protein Ag2149 种属同源性预测
Host / Isotype Mouse / IgG2a
Class Monoclonal
Type Antibody
Full Name RAS and EF-hand domain containing
Synonyms FLJ31614, RAB45, Ras related protein Rab 45, RASEF
Calculated Molecular Weight 740 aa, 83 kDa
Observed Molecular Weight 90 kDa
GenBank Accession NumberBC023566
Gene Symbol RASEF
Gene ID (NCBI) 158158
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
UNIPROT IDQ8IZ41
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

The RASEF (RAS and EF-hand domain-containing protein), a member of Rab GTPase protein family, contains a Rab GTPase domain in the C-terminal region, a coiled coil motif in an internal region as well as 2 EF hand domains which were of importance for binding to calcium ions in the N-terminus.