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RASGEF1B Polyclonal antibody

RASGEF1B Polyclonal Antibody for IHC, WB,ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

IHC, WB,ELISA

Conjugate

Unconjugated

Cat No : 13617-1-AP

Print datasheet

Synonyms

GPI gamma 4, GPIG4, RASGEF1B

验证数据展示

  • WB analysis of human brain using 13617-1-AP

    WB analysis of human brain using 13617-1-AP

    human brain tissue were subjected to SDS PAGE followed by western blot with 13617-1-AP (RASGEF1B antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

  • IHC staining of human stomach using 13617-1-AP

    IHC staining of human stomach using 13617-1-AP

    Immunohistochemical analysis of paraffin-embedded human stomach using 13617-1-AP (RASGEF1B antibody) at dilution of 1:50 (under 10x lens).

  • IHC staining of human stomach using 13617-1-AP

    IHC staining of human stomach using 13617-1-AP

    Immunohistochemical analysis of paraffin-embedded human stomach using 13617-1-AP (RASGEF1B antibody) at dilution of 1:50 (under 40x lens).

经过测试的应用

Positive WB detected inhuman brain tissue, mouse lung tissue, mouse ovary tissue
Positive IHC detected inhuman stomach tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
Immunohistochemistry (IHC)IHC : 1:20-1:200
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

13617-1-AP targets RASGEF1B in WB, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Applications IHC, WB,ELISA Application Description
Tested Reactivity human, mouse, rat
Immunogen RASGEF1B fusion protein Ag4525 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name RasGEF domain family, member 1B
Synonyms GPI gamma 4, GPIG4, RASGEF1B
Calculated Molecular Weight 55 kDa
Observed Molecular Weight 50 kDa
GenBank Accession NumberBC036784
Gene Symbol RASGEF1B
Gene ID (NCBI) 153020
RRIDAB_10644147
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ0VAM2
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

RASGEF1B is a guanine-nucleotide exchange factor, whose expression is induced inmacrophages on stimulation by TLR2 and TLR4 agonists. It interacts with Ras family proteins. It also interacts with CCDC124 during cytokinesis.

实验方案

Product Specific Protocols
WB protocol for RASGEF1B antibody 13617-1-APDownload protocol
IHC protocol for RASGEF1B antibody 13617-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols