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RASGRP2 Polyclonal antibody

RASGRP2 Polyclonal Antibody for WB, FC (Intra), ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, FC (Intra), ELISA and More (1)

Conjugate

Unconjugated

Cat no : 30189-1-AP

Print datasheet

Synonyms

GRP2, F25B3.3 kinase-like protein, Cdc25-like protein, CDC25L, CalDAG-GEFI

验证数据展示

  • WB analysis using 30189-1-AP

    WB analysis using 30189-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 30189-1-AP (RASGRP2 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

  • FC experiment of Jurkat using 30189-1-AP

    FC experiment of Jurkat using 30189-1-AP

    1X10^6 Jurkat cells were intracellularly stained with 0.4 ug Anti-Human RASGRP2 (30189-1-AP) and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) at dilution 1:1000 (red), or 0.4 ug Control Antibody. Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

经过测试的应用

Positive WB detected inJurkat cells, MOLT-4 cells, Raji cells
Positive FC (Intra) detected inJurkat cells
Planning an IF experiment? We recommend our CoraLite® Plus 488 conjugated versions of this antibody.

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:6000
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

30189-1-AP targets RASGRP2 in WB, FC (Intra), CoIP, ELISA applications and shows reactivity with human samples.

Tested Applications WB, FC (Intra), ELISA Application Description
Cited ApplicationsWB, CoIP
Tested Reactivity human
Cited Reactivityhuman
Immunogen RASGRP2 fusion protein Ag32762 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name RAS guanyl releasing protein 2 (calcium and DAG-regulated)
Synonyms GRP2, F25B3.3 kinase-like protein, Cdc25-like protein, CDC25L, CalDAG-GEFI
Calculated Molecular Weight69kd
Observed Molecular Weight69 kDa
GenBank Accession NumberNM_153819
Gene Symbol RASGRP2
Gene ID (NCBI) 10235
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ7LDG7
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

RASGRP2, also named as CDC25L and MCG7, belongs to the RASGRP family. It functions as a calcium- and DAG-regulated nucleotide exchange factor specifically activating Rap through the exchange of bound GDP for GTP. RASGRP2 may also activate other GTPases such as RRAS, RRAS2, NRAS, KRAS but not HRAS. RASGRO2 functions in aggregation of platelets and adhesion of T-lymphocytes and neutrophils probably through inside-out integrin activation. It may function in the muscarinic acetylcholine receptor M1/CHRM1 signaling pathway. The antibody recognizes the C-term of RASGRP2.

实验方案

Product Specific Protocols
WB protocol for RASGRP2 antibody 30189-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
humanWB,CoIP

Biochim Biophys Acta Mol Cell Res

NEDD4L-mediated RASGRP2 suppresses high-glucose and oxLDL-induced vascular endothelial cell dysfunctions by activating Rap1 and R-Ras

Authors - Guozhu Chen
View Article