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CoraLite® Plus 488-conjugated RASGRP2 Polyclonal antibody

RASGRP2 Polyclonal Antibody for FC (Intra)

Host / Isotype

Rabbit / IgG

Reactivity

Human

Applications

FC (Intra)

Conjugate

CoraLite® Plus 488 Fluorescent Dye

Cat No : CL488-30189

Print datasheet

Synonyms

CALDAG GEFI, Cdc25 like protein, CDC25L, F25B3.3 kinase like protein, hCDC25L, MCG7, RAS guanyl releasing protein 2, RAS-GRP, RASGRP2

验证数据展示

  • FC experiment of Jurkat using CL488-30189

    FC experiment of Jurkat using CL488-30189

    1x10^6 Jurkat cells were intracellularly stained with 0.8 ug Coralite® Plus 488 Rasgrp2 Polyclonal Antibody (CL488-30189)(red), or 0.8 ug CoraLite® Plus 488-conjugated Rabbit IgG control Rabbit PolyAb (CL488-30000) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

经过测试的应用

Positive FC detected inJurkat cells
For other applications, we recommend the unconjugated version of this antibody, 30189-1-AP

推荐稀释比

ApplicationDilution
Flow Cytometry (FC)FC : 0.80 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

CL488-30189 targets RASGRP2 in FC (Intra) applications and shows reactivity with Human samples.

Tested Applications FC (Intra) Application Description
Tested Reactivity Human
Immunogen RASGRP2 fusion protein Ag32762 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name RAS guanyl releasing protein 2 (calcium and DAG-regulated)
Synonyms CALDAG GEFI, Cdc25 like protein, CDC25L, F25B3.3 kinase like protein, hCDC25L, MCG7, RAS guanyl releasing protein 2, RAS-GRP, RASGRP2
Calculated Molecular Weight69kd
Observed Molecular Weight69 kDa
GenBank Accession NumberNM_153819
Gene Symbol RASGRP2
Gene ID (NCBI) 10235
Conjugate CoraLite® Plus 488 Fluorescent Dye
Excitation/Emission Maxima Wavelengths493 nm / 522 nm
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ7LDG7
Storage Buffer PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
Storage ConditionsStore at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

RASGRP2, also named as CDC25L and MCG7, belongs to the RASGRP family. It functions as a calcium- and DAG-regulated nucleotide exchange factor specifically activating Rap through the exchange of bound GDP for GTP. RASGRP2 may also activate other GTPases such as RRAS, RRAS2, NRAS, KRAS but not HRAS. RASGRO2 functions in aggregation of platelets and adhesion of T-lymphocytes and neutrophils probably through inside-out integrin activation. It may function in the muscarinic acetylcholine receptor M1/CHRM1 signaling pathway. The antibody recognizes the C-term of RASGRP2.

实验方案

Product Specific Protocols
FC protocol for CL Plus 488 RASGRP2 antibody CL488-30189Download protocol
Standard Protocols
Click here to view our Standard Protocols